Here we report the catching of a single specimen of a rare native fish species, the cactus roach Rutilus virgo (Heckel), by rod and line on 31 January 2016 at Břeclav (r.km 25.6), on the main channel of the River Dyje (right tributary of the River Morava, Danube basin). This report comes 62-years after the last confirmed record in the Czech Republic, the species having been reported as extinct in the country since then. The morphometric and genetic characteristics of the specimen are presented and evaluated in light of the previous report.