Stable carbon and nitrogen isotope analyses of bones and teeth at the ancestral heritage Muwekma Ohlone site of Yakmuy ´Ooyákma-tka (“Place of the East Ridge Site”; CA-SCL-215) reveal significant differences in the dietary life history of males and females. Overall, isotope data indicate that site inhabitants were primarily dependent on low-trophic-level foods, likely plants, and minor amounts of marine food for their main source of dietary protein. From tooth dentin serial samples, we found that males and females were similar for δ¹³C in early childhood (age 1–9 years), but boys were elevated in δ¹⁵N by 0.6–1.0‰ relative to girls, indicating boys were accessing slightly greater amounts of higher-trophic-level foods, such as meat from game. The sex-biased difference in δ¹⁵N diminishes during the second decade of life, as female δ¹⁵N values increase and become equal to males. However, a difference in δ¹³C emerges during the second decade: female δ¹³C values are elevated relative to males. This could indicate that teenage females consumed higher amounts of low-trophic-level, marine-derived protein, such as shellfish. During later adult years the difference in δ¹³C disappears, while males again show an increase in δ¹⁵N relative to females. Although these differences are small, they reveal important sex-biased life history patterns during childhood and adulthood in this ancient community.