A cell line derived from the larval-fat body tissues of the wax moth, Galleria mellonella Linne, was established in MGM-450 medium. The cells grew in suspension and were mainly spherical in shape. Population doubling time was between 1.4 and 1.7 d over a range of 15 to 35° C, and the maximum growth rate was at 25° C. The chromosome number ranged from 70–239, with a mode of 170. The cells were sensitive to 20-hydroxyecdysone, which stimulated their growth and induced morphological changes. The cell line was designated GaMe-LF1.
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1 January 2006
A NEW CELL LINE FROM THE WAX MOTH GALLERIA MELLONELLA LINNE (LEPIDOPTERA: PYRALIDIDAE)
DAISUKE EGUCHI,
KIKUO IWABUCHI
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In Vitro Cellular & Developmental Biology - Animal
Vol. 42 • No. 1
January 2006
Vol. 42 • No. 1
January 2006
Cell line
fat body
Galleria mellonella