Fetal calf serum (FCS) is conventionally used for animal cell cultures due to its inherent growth-promoting activities. However animal welfare issues and stringent requirements for human transplantation studies demand a suitable alternative for FCS. With this view, we studied the effect of FCS, human AB serum (ABS), and human umbilical cord blood serum (UCBS) on murine islets of Langerhans and human bone marrow–derived mesenchymal-like cells (hBMCs). We found that there was no difference in morphology and functionality of mouse islets cultured in any of these three different serum supplements as indicated by insulin immunostaining. A comparative analysis of hBMCs maintained in each of these three different serum supplements demonstrated that UCBS supplemented media better supported proliferation of hBMCs. Moreover, a modification of adipogenic differentiation protocol using UCBS indicates that it can be used as a supplement to support differentiation of hBMCs into adipocytes. Our results demonstrate that UCBS not only is suitable for maintenance of murine pancreatic islets, but also supports attachment, propagation, and differentiation of hBMCs in vitro. We conclude that UCBS can serve as a better serum supplement for growth, maintenance, and differentiation of hBMCs, making it a more suitable supplement in cell systems that have therapeutic potential in human transplantation programs.
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1 November 2006
HUMAN UMBILICAL CORD BLOOD SERUM PROMOTES GROWTH, PROLIFERATION, AS WELL AS DIFFERENTIATION OF HUMAN BONE MARROW–DERIVED PROGENITOR CELLS
SMRUTI M. PHADNIS,
MUGDHA V. JOGLEKAR,
VIJAYALAKSHMI VENKATESHAN,
SURENDRA M. GHASKADBI,
ANANDWARDHAN A. HARDIKAR,
RAMESH R. BHONDE
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In Vitro Cellular & Developmental Biology - Animal
Vol. 42 • No. 10
November 2006
Vol. 42 • No. 10
November 2006
Bone marrow
fetal calf serum
Islets
serum supplement
umbilical cord blood serum