A continuous cell line, PBLE, was developed from the adherent cells in a culture of peripheral blood leukocytes from the American eel, Anguilla rostrata. The cells were grown in Leibovitz's L-15 basal medium supplemented with 20% fetal bovine serum (FBS). Under normal culture conditions at 18° C, the morphology of PBLE was fibroblast-like. The cultures have been subcultured over 80 times and have been cryopreserved successfully. These cells have a diploid karyotype of 38 chromosomes, survived temperatures from 5 to 36° C, and proliferated at temperatures from 5° C to at least 30° C. PBLE underwent apoptosis in response to gliotoxin, but did not show a respiratory burst. Results suggest that PBLE may have arisen from a circulating mesenchymal stem cell. PBLE was susceptible to Chum salmon reovirus (CSV) and supported CSV replication. Therefore this cell line should be useful in studying eel specific virus–host interactions.
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1 September 2006
DEVELOPMENT OF A CONTINUOUS CELL LINE, PBLE, FROM AN AMERICAN EEL PERIPHERAL BLOOD LEUKOCYTE PREPARATION
S. J. DEWITTE-ORR,
K. LEPIC,
S. P. BRYSON,
S. K. WALSH,
L. E. J. LEE,
N. C. BOLS
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In Vitro Cellular & Developmental Biology - Animal
Vol. 42 • No. 8
September 2006
Vol. 42 • No. 8
September 2006
Anguilla rostrata
Apoptosis
gliotoxin
reovirus
Stem cell
temperature tolerance