Hairy roots of Brugmansia candida were exposed to different elicitors, such as pectinase, B. candida root homogenate, Hormonema ssp. homogenate, and the acetate control buffer. Pectinase increased intracellular hyoscyamine (200–300%) and the release of both alkaloids up to 1500% (scopolamine) and 1100% (hyoscyamine). However, the increment observed in both alkaloids in roots with the acetate control buffer was superior than with pectinase, obtaining increases of 700% in hyoscyamine and 200% in scopolamine. The B. candida root homogenate enhanced the accumulation (50–600%) and specific production of both alkaloids (ca. 150%). Hormonema ssp. homogenates induced different responses according to the original medium in which the fungus was cultured. The effect of each elicitor is discussed.
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1 November 2003
COMPARISON OF THE INFLUENCE OF DIFFERENT ELICITORS ON HYOSCYAMINE AND SCOPOLAMINE CONTENT IN HAIRY ROOT CULTURES OF BRUGMANSIA CANDIDA
S. PITTA ALVAREZ,
P. L. MARCONI,
A. GIULIETTI
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In Vitro Cellular and Developmental Biology - Plant
Vol. 39 • No. 6
November 2003
Vol. 39 • No. 6
November 2003
biotic elicitors
Brugmansia candida
hyoscyamine
scopolamine
tropane alkaloids