INFLUENCE OF TRIACONTANOL ON SOMATIC EMBRYOGENESIS IN COFFEA ARABICA L. AND COFFEA CANEPHORA P. EX FR.

P. GIRIDHAR; E. P. INDU; G. A. RAVISHANKAR; A. CHANDRASEKAR

doi:10.1079/IVP2003519

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1 March 2004 INFLUENCE OF TRIACONTANOL ON SOMATIC EMBRYOGENESIS IN COFFEA ARABICA L. AND COFFEA CANEPHORA P. EX FR.

P. GIRIDHAR, E. P. INDU, G. A. RAVISHANKAR, A. CHANDRASEKAR

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In Vitro Cellular and Developmental Biology - Plant, 40(2):200-203 (2004). https://doi.org/10.1079/IVP2003519

Abstract

A highly reproducible method for regeneration of Coffea arabica and C. canephora plants via direct somatic embryogenesis from cultured leaf and stem segments of regenerated plants was developed. Embryogenesis was influenced by the presence of triacontanol (TRIA) in the medium. TRIA incorporated at 4.55 and 11.38 μM in half-strength MS basal medium containing 1.1 μM 6-benzyladenine (BA) and 2.28 μM indole-3-acetic acid (IAA) induced direct somatic embryogenesis in both species. A maximum of 260 ± 31.8 and 59.2 ± 12.8 somatic embryos per culture were induced from in vitro leaf explants of C. arabica and C. canephora, respectively. TRIA also induced embryo formation from in vitro stem segment callus tissues along with multiplication of primary embryos into secondary embryos. By using TRIA, it was possible to obtain somatic embryogenesis in C. arabica and C. canephora.

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P. GIRIDHAR, E. P. INDU, G. A. RAVISHANKAR, and A. CHANDRASEKAR "INFLUENCE OF TRIACONTANOL ON SOMATIC EMBRYOGENESIS IN COFFEA ARABICA L. AND COFFEA CANEPHORA P. EX FR.," In Vitro Cellular and Developmental Biology - Plant 40(2), 200-203, (1 March 2004). https://doi.org/10.1079/IVP2003519

Received: 2 July 2003; Accepted: 1 November 2003; Published: 1 March 2004

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