A new protocol has been developed for the highly efficient somatic embryogenesis and plant regeneration of 10 recalcitrant Chinese cotton cultivars. Calluses and embryogenic calluses were induced on MSB1 medium containing the optimal combination of indolebutyric acid (IBA; 2.46 μM) and kinetin (KT; 2.32 μM). Up to 86.7% of embryogenic calluses differentiated into globular somatic embryos 2 mo. after culture on MSB2 medium containing double KNO3 and free of growth regulators. Up to 38.3% of the somatic embryos were converted into complete plants in 8 wk on MSB3 medium with l-asparagine (Asn)/l-glutamine (Gln) (7.6/13.6 mM). The plants were successfully transferred to soil and grew to maturity. With the protocol described here, we have obtained hundreds of regenerating plantlets from 10 recalcitrant cultivars, which is important for the application of tissue culture to cotton breeding and biotechnology.
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1 July 2004
FACTORS AFFECTING SOMATIC EMBRYOGENESIS AND PLANT REGENERATION FROM A RANGE OF RECALCITRANT GENOTYPES OF CHINESE COTTONS (GOSSYPIUM HIRSUTUM L)
JIAHE WU,
XIANLONG ZHANG,
YICHUN NIE,
SHUANGXIA JIN,
SHAOGUANG LIANG
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In Vitro Cellular and Developmental Biology - Plant
Vol. 40 • No. 4
July 2004
Vol. 40 • No. 4
July 2004
cotton
IBA
in vitro culture
recalcitrant genotypes
Somatic embryos