The effect of abscisic acid (ABA) was evaluated during the maturation and germination of holm oak (Quercus ilex L.) somatic embryos. The addition of ABA to the culture medium significantly reduced unwanted recurrent embryogenesis in mature somatic embryos without affecting the germination of embryos subjected to stratification at 4°C. Stratification at 4°C for 2 mo. was the most efficient for stimulating somatic embryo germination of holm oak. The addition of 90 and 450 mM sucrose also improved germination, while higher sucrose concentrations were inhibitory.
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1 September 2004
EFFECT OF ABSCISIC ACID AND STRATIFICATION ON SOMATIC EMBRYO MATURATION AND GERMINATION OF HOLM OAK (QUERCUS ILEX L)
P. V. MAURI,
J. A. MANZANERA
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In Vitro Cellular and Developmental Biology - Plant
Vol. 40 • No. 5
September 2004
Vol. 40 • No. 5
September 2004
ABA
in vitro
somatic embryogenesis
sucrose