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1 September 2004 EFFECT OF ABSCISIC ACID AND STRATIFICATION ON SOMATIC EMBRYO MATURATION AND GERMINATION OF HOLM OAK (QUERCUS ILEX L)
P. V. MAURI, J. A. MANZANERA
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Abstract

The effect of abscisic acid (ABA) was evaluated during the maturation and germination of holm oak (Quercus ilex L.) somatic embryos. The addition of ABA to the culture medium significantly reduced unwanted recurrent embryogenesis in mature somatic embryos without affecting the germination of embryos subjected to stratification at 4°C. Stratification at 4°C for 2 mo. was the most efficient for stimulating somatic embryo germination of holm oak. The addition of 90 and 450 mM sucrose also improved germination, while higher sucrose concentrations were inhibitory.

P. V. MAURI and J. A. MANZANERA "EFFECT OF ABSCISIC ACID AND STRATIFICATION ON SOMATIC EMBRYO MATURATION AND GERMINATION OF HOLM OAK (QUERCUS ILEX L)," In Vitro Cellular and Developmental Biology - Plant 40(5), 495-498, (1 September 2004). https://doi.org/10.1079/IVP2004557
Received: 19 February 2003; Accepted: 1 April 2004; Published: 1 September 2004
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KEYWORDS
ABA
in vitro
somatic embryogenesis
sucrose
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