Shoot tips and leaves excised from in vitro shoot cultures of Salvia nemorosa were evaluated for their organogenic capacity under in vitro conditions. The best shoot proliferation from shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with 8.9 μM 6-benzylaminopurine (BA) and 2.9 μM indole-3-acetic acid (IAA). Leaf lamina and petiole explants formed shoots through organogenesis via callus stage and/or directly from explant tissue. The highest values for shoot regeneration were obtained with 0.9 μM BA and 2.9 μM IAA for lamina explants. No shoot organogenesis was obtained on leaf explants cultured on MS medium supplemented with α-naphthaleneacetic acid (NAA). The regenerated shoots rooted the best on MS medium containing 0.6 μM IAA or 0.5 μM NAA. In vitro-propagated plants were transferred to soil with a survival rate of 85% after 3 mo.
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1 November 2004
IN VITRO REGENERATION OF SALVIA NEMOROSA L. FROM SHOOT TIPS AND LEAF EXPLANTS
E. W A. SKAłA,
H. A L. I N. A. WYSOKIŃSKA
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In Vitro Cellular and Developmental Biology - Plant
Vol. 40 • No. 6
November 2004
Vol. 40 • No. 6
November 2004
adventitious shoots
Hyperhydricity
micropropagation
Organogenesis