A method was developed for plant regeneration from alginate-encapsulated shoot tips of Phyllanthus amarus. Shoot tips excised from in vitro proliferated shoots were encapsulated in calcium alginate beads. The best gel complexation was achieved using 3% sodium alginate and 75 mM CaCl2·2H2O. Maximum percentage response for conversion of encapsulated shoot tips into plantlets was 90% after 5 wk of culture on Murashige and Skoog (MS) medium without plant growth regulator. The regrowth ability of encapsulated shoot tips was affected by the concentration of sodium alginate, storage duration, and the presence or absence of MS nutrients in calcium alginate beads. Plantlets with well-developed shoot and roots were transferred to pots containing an autoclaved mixture of soilrite and peat moss (1:1). The conversion of encapsulated shoot tips into plantlets also occurred when calcium alginate beads were directly sown in autoclaved soilrite moistened with 1/4-MS salts. Encapsulation of vegetative propagules in calcium alginate beads can be used as an alternative to synthetic seeds derived from somatic embryos.
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1 March 2006
PLANT REGENERATION FROM ALGINATE-ENCAPSULATED SHOOT TIPS OF PHYLLANTHUS AMARUS SCHUM AND THONN, A MEDICINALLY IMPORTANT PLANT SPECIES
A. K. SINGH,
M. SHARMA,
R. VARSHNEY,
S. S. AGARWAL,
K. C. BANSAL
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In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 2
March 2006
Vol. 42 • No. 2
March 2006
alginate beads
encapsulation
Phyllanthus amarus
Shoot tip
synthetic seed