A micropropagation protocol for Pouteria lucuma R. and Pav. var. La Molina was developed. Shoots from zygotic embryos with a portion of endosperm were established in vitro on Murashige and Skoog (MS) medium with 0.47 μM kinetin (Kin) and 0.54 μM naphthaleneacetic acid (NAA). Multiplication of shoots was accomplished using subapical shoots. The best axillary-shoot production was observed on MS basal medium with 2.2 μM benzyladenine (BA), 0.5 μM NAA, 1.4 μM gibberellic acid (GA3), and 40 mg l−1 adenine sulfate, with the development of up to three axillary shoots per subapical shoot. One hundred percent rooting was obtained from shoots grown for 4 wk on MS medium with 246 μM indole-3-butyric acid under light conditions. Eighty percent of the microplantlets survived after acclimatization when transplanted to a substrate previously enriched with beneficial soil bacteria. This study describes, for the first time, arbuscular mycorrhizal (AM) colonization of this species. Inoculation with AM fungi improved growth and development of lucumo plants and induced changes to the root morphology.
How to translate text using browser tools
1 March 2006
MICROPROPAGATION AND EFFECTS OF MYCORRHIZA AND SOIL BACTERIA ON ACCLIMATIZATION AND DEVELOPMENT OF LUCUMO (POUTERIA LUCUMA R. AND PAV.) VAR. LA MOLINA
I. M. G. PADILLA,
E. CARMONA,
N. WESTENDORP,
C. L. ENCINA
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 2
March 2006
Vol. 42 • No. 2
March 2006
Acclimatization
bacterial colonization
cloning
in vitro culture
multiplication
rooting, Sapotaceae