Indirect organogenesis was developed in Agave tequilana. Leaf segments and meristematic tissue from the central head (‘piña’) were evaluated as explant sources. A minimal-sized explant with high bud-forming capacity (19.5 BFC) was obtained through a cross section of meristematic tissue from in vitro plantlets. In callus culture, the best growth response was due to naphthalene acetic-acid (NAA) presenting a contrasting response compared to 2,4-dichlorophenoxyacetic acid (2,4-D). Regeneration from meristem segments and callus was obtained using 1.1 μM 2,4-D and 44 μM 6-benzylaminopurine (BA). The regeneration capacity of callus was maintained for 3 mo. Shoots regenerated were rooted in a hormone-free MS1 medium and acclimatized in a greenhouse with a 100% survival.
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1 July 2006
PLANT REGENERATION OF AGAVE TEQUILANA BY INDIRECT ORGANOGENESIS
KARLA K. VALENZUELA-SÁNCHEZ,
RAUL E. JUÁREZ-HERNÁNDEZ,
ANDRÉS CRUZ-HERNÁNDEZ,
VÍCTOR OLALDE-PORTUGAL,
MARÍA ELENA VALVERDE,
OCTAVIO PAREDES-LÓPEZ
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In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 4
July 2006
Vol. 42 • No. 4
July 2006
Agavaceae
Auxins
callus culture
meristem
Tissue culture