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1 July 2006 PLANT REGENERATION OF AGAVE TEQUILANA BY INDIRECT ORGANOGENESIS
KARLA K. VALENZUELA-SÁNCHEZ, RAUL E. JUÁREZ-HERNÁNDEZ, ANDRÉS CRUZ-HERNÁNDEZ, VÍCTOR OLALDE-PORTUGAL, MARÍA ELENA VALVERDE, OCTAVIO PAREDES-LÓPEZ
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Abstract

Indirect organogenesis was developed in Agave tequilana. Leaf segments and meristematic tissue from the central head (‘piña’) were evaluated as explant sources. A minimal-sized explant with high bud-forming capacity (19.5 BFC) was obtained through a cross section of meristematic tissue from in vitro plantlets. In callus culture, the best growth response was due to naphthalene acetic-acid (NAA) presenting a contrasting response compared to 2,4-dichlorophenoxyacetic acid (2,4-D). Regeneration from meristem segments and callus was obtained using 1.1 μM 2,4-D and 44 μM 6-benzylaminopurine (BA). The regeneration capacity of callus was maintained for 3 mo. Shoots regenerated were rooted in a hormone-free MS1 medium and acclimatized in a greenhouse with a 100% survival.

KARLA K. VALENZUELA-SÁNCHEZ, RAUL E. JUÁREZ-HERNÁNDEZ, ANDRÉS CRUZ-HERNÁNDEZ, VÍCTOR OLALDE-PORTUGAL, MARÍA ELENA VALVERDE, and OCTAVIO PAREDES-LÓPEZ "PLANT REGENERATION OF AGAVE TEQUILANA BY INDIRECT ORGANOGENESIS," In Vitro Cellular and Developmental Biology - Plant 42(4), 336-340, (1 July 2006). https://doi.org/10.1079/IVP2006788
Received: 18 July 2005; Accepted: 1 May 2006; Published: 1 July 2006
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KEYWORDS
Agavaceae
Auxins
callus culture
meristem
Tissue culture
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