The subfamily Phimophorinae includes ∼113 species of cryptic assassin bugs found in the Neotropics and Paleotropics. Presumably due to its small size, cryptic coloration, and occurrence in the remote Eastern Arc Mountains (EAM), Porcelloderes impenetrabilis Rédei, 2012 (Heteroptera: Reduviidae: Phimophorinae), was only recently described as the first representative of this subfamily from mainland Africa. Previous morphology-based phylogenetic research on Phimophorinae (then known as Physoderinae or Epiroderinae) recovered Porcelloderes as sister taxon to the remaining epiroderine-lineage of Phimophorinae. Recent fieldwork in Tanzania has resulted in 241 additional specimens of Porcelloderes from 3 EAM) ranges. We used molecular and morphological analyses for these 241 specimens to (i) determine their species identity, (ii) test the phylogenetic position of Porcelloderes within Phimophorinae, and (iii) determine if divergence within Porcelloderes coincides with periods of climate-driven forest fragmentation in the EAM. Specimens from the Kimboza and Nguru Mountains likely represent P. impenetrabilis, but we here describe Porcelloderes harles, n. sp., from the Udzungwa Mountains. Our molecular phylogeny shows Porcelloderes nested within the epiroderine-lineage of Phimophorinae and as sister taxon to a Madagascar clade. We estimate that the epiroderine-lineage diverged from other Phimophorinae ∼44 MYA.The divergence estimates for the 2 Porcelloderes species is ∼15 MYA, consistent with proposed dates for other EAM assassin bugs and a period of forest fragmentation.

Graphical Abstract