The objective of this study was to establish hematological and plasma biochemical reference values in captive white-fronted Amazon parrots (Amazona albifrons), as well as to determine whether sex effects the reference values. To our knowledge, hematological and plasma biochemical data have not been reported in this species. Thirty-seven clinically healthy adult individuals (21 males, 16 females) from El Nido Bird Sanctuary, Ixtapaluca, Estado de México, were the subject birds for this study. Complete blood count and selected plasma biochemical parameters, including uric acid, glutamate dehydrogenase, aspartate aminotransferase, total protein, and albumin, were evaluated. Blood samples were collected in the winter (January), outside of the birds' breeding season. Many hematological and plasma biochemical analytes had large coefficients of variation, and there were no statistically significant sex differences identified.