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14 October 2015 Managing Ammonia Emissions From Screwworm Larval Rearing Media
Agustin Sagel, Pamela Phillips, Muhammad Chaudhury, Steven Skoda
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Abstract

Mass production, sterilization, and release of screwworms (Cochliomyia hominivorax (Coquerel)) that were competitive in the field significantly contributed to the successful application of the sterile insect technique for eradication of screwworms from continental North America. Metabolic byproducts resulting from protein-rich diets required for larval screwworms lead to ammonia liberation, sometimes at high levels, within the mass rearing facility. Until recently a sodium polyacrylate gel bulking agent was used for the larval media and adsorbed much of the ammonia. A need to replace the gel with an environmentally "friendly" bulking agent, while not increasing ammonia levels in the rearing facility, led to a series of experiments with the objective of developing procedures to reduce ammonia emissions from the larval media bulked with cellulose fiber. Additives of ammonia-converting bacteria, potassium permanganate, and Yucca schidigera Roezl ex Otrgies powder extract, previously reported to reduce ammonia levels in organic environments, were evaluated. Ammonia-converting bacteria did not have a positive effect. Addition of Y. schidigera powder extract (∼1% of total volume), potassium permanganate (∼250 ppm), and a combination of these two additives (at these same concentrations) kept ammonia at equivalent levels as when larval media was bulked with gel. Potassium permanganate also had sufficient antimicrobial properties that the use of formaldehyde in the diet was not necessary. Further testing is needed, at a mass rearing level, before full implementation into the screwworm eradication program.

Published by Oxford University Press on behalf of Entomological Society of America 2015. This work is written by US Government employees and is in the public domain in the US.
Agustin Sagel, Pamela Phillips, Muhammad Chaudhury, and Steven Skoda "Managing Ammonia Emissions From Screwworm Larval Rearing Media," Journal of Economic Entomology 109(1), 478-483, (14 October 2015). https://doi.org/10.1093/jee/tov291
Received: 9 July 2015; Accepted: 9 September 2015; Published: 14 October 2015
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KEYWORDS
cellulose fiber
Cochliomyia hominivorax
insect mass rearing
potassium permanganate
sterile insect technique
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