Striacosta albicosta (Smith; Lepidoptera: Noctuidae) is a pest of corn (Zea mays L.), which has recently expanded its range into Ontario, Canada. Genetically modified corn expressing Vip3A insecticidal protein from Bacillus thuringiensis is a biotechnological option for the control of S. albicosta.To support an insect resistance management program, we conducted a study of baseline susceptibility of 10-field collected S. albicosta populations in Ontario, Canada to Vip3A before widespread commercial adoption. Neonates were exposed to artificial diet overlaid with Vip3A. The LC₅₀ ranged from 22.7 to 53.5 ng Vip3A cm^–2. The EC₅₀ ranged from 11.4 to 30.2 ng Vip3A cm^–2. There was low inter-population variation in susceptibility to Vip3A, which we believe represents the natural geographical variation in response and not variation caused by previous exposure to selection pressure of the Vip3A protein.