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5 March 2018 New Insights into the Methylation Status of Virulent Diuraphis noxia (Hemiptera: Aphididae) Biotypes
Kelly Breeds, N. Francois V. Burger, Anna-Maria Botha
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Abstract

Epigenetic modifications provide a means for aphid biotype development that a lack of genetic variation, owing to an anholocyclic reproduction lifecycle, fails to do. Here we present data on the DNA methylation status in four South African Russian wheat aphids (RWA), Diuraphis noxia (Kurdjomov) (Hemiptera: Aphididae) biotypes expressing different levels of virulence against its host, Triticum aestivum L. (Poales: Poaceae, Triticeae). The DNA methylation status of these biotypes was determined through the use of methylation-sensitive amplification polymorphism analysis, restriction site-specific fluorescence labeling–a novel technique, and measuring relative global DNA methylation and hydroxymethylation. The least virulent RWA biotype, SA1, was methylated, while biotypes displaying intermediate virulence, SA2 and SA3, exhibited intermediate levels of hemimethylation. The genome of the most virulent RWA biotype, SAM, seems to be hypomethylated, which is likely attained through the process of demethylation.

© The Author(s) 2018. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Kelly Breeds, N. Francois V. Burger, and Anna-Maria Botha "New Insights into the Methylation Status of Virulent Diuraphis noxia (Hemiptera: Aphididae) Biotypes," Journal of Economic Entomology 111(3), 1395-1403, (5 March 2018). https://doi.org/10.1093/jee/toy039
Received: 16 October 2017; Accepted: 26 January 2018; Published: 5 March 2018
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KEYWORDS
biotypification
hemimethylation
Hemiptera: Aphididae
hypermethylation
MSAP
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