A molecular method is presented for differentiating the morphologically cryptic leafminers Liriomyza langei Frick and L. huidobrensis (Blanchard). This method requires polymerase chain reaction (PCR) amplification of a 1031-bp region of mitochondrial cytochrome oxidase DNA followed by restriction fragment analysis using the restriction enzymes SpeI and EcoRV. SpeI cuts the mitochondrial fragment of L. langei into two fragments, but does not cut the L. huidobrensis fragment. EcoRV cuts the L. huidobrensis fragment into two fragments, but does not cut the L. langei fragment. This PCR-restriction fragment-length polymorphism (RFLP) method is faster and less costly than DNA sequencing, which is currently the only other way to differentiate these two species. We apply the method to samples from recently introduced leafminer populations in Sri Lanka, Canada, and South Africa and find that the invasive leafminer in all three locations is L. huidobrensis.
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1 October 2001
Polymerase Chain Reaction-Restriction Fragment-Length Polymorphism Method to Distinguish Liriomyza huidobrensis from L. langei (Diptera: Agromyzidae) Applied to Three Recent Leafminer Invasions
Sonja J. Scheffer,
Anura Wijesekara,
Diedrich VISSER,
Rebecca h. Hallett
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Journal of Economic Entomology
Vol. 94 • No. 5
October 2001
Vol. 94 • No. 5
October 2001
Agromyzidae
introduced species
invasive species
molecular identification
pea leafminer
polymerase chain reaction-restriction fragment-length polymorphism