The red imported fire ant, Solenopsis invicta Buren, is an abundant predator in cropping systems throughout its range. It has been documented to be an important predator of numerous crop pests, as well as being an agricultural pest itself. Information on the impact of insecticides on natural enemies such as fire ants is necessary for the integration of biological and chemical control tactics in an effective pest management program. Therefore, a residual vial bioassay was developed to determine the concentration-mortality responses of S. invicta workers to four commonly used insecticides: acephate, chlorpyrifos, methomyl, and lambda-cyhalothrin. Fire ant workers showed a mortality response to serial dilutions to all four chemicals. Methomyl (LC₅₀ 0.04 μg/vial, fiducial limits 0.03–0.06) was the most toxic, followed by chlorpyrifos (LC₅₀ 0.11 μg/vial, fiducial limits 0.07–0.17) and acephate (LC₅₀ 0.76 μg/vial, fiducial limits 0.50–1.04). Of the chemicals assayed, it took a much higher concentration of lambda-cyhalothrin (LC₅₀ 2.30 μg/vial, fiducial limits 1.57–3.59) to kill 50% of the workers compared with the other three chemicals. The results of this study demonstrate the wide range in responses of fire ants to four insecticides that are labeled and commonly used on numerous agricultural crops throughout the United States. These results further suggest the possibility of using a discriminating dose of lambda-cyhalothrin to control the target pest species while conserving fire ants in the agricultural systems in which their predatory behavior is beneficial to the integrated pest management program.