Twenty-two species collected with Malaise traps are recorded for the first time from Spain. These are: Agromyza anthracina Meigen, 1830; A. bromi Spencer, 1966; A. hiemalis Becker, 1908; A. megalopsis Hering, 1933; Aulagromyza buhri(de Meijere, 1938); Au. luteoscutellata (de Meijere, 1924); Au. similis (Brischke, 1880); Au. trivitatta (Loew, 1873); Cerodontha (Poemyza) lapplandica (Rydén, 1956); Liriomyza amoena (Meigen, 1830); L. erucifolii de Meijere, 1944; L. graminivora Hering, 1949; L. samogitica Pakalniškis, 1996; Melanagromyza eupatorii Spencer, 1957; M. nibletti Spencer, 1957; M. spinulosa Spencer, 1974; Ophiomyia labiatarum Hering, 1937; O. penicillata Hendel, 1920; Phytobia cerasiferae (Kangas, 1955); P. lunulata (Hendel, 1920); Phytomyza bupleuri Hering, 1963; and Ph. tanaceti Hendel, 1923. These new reports increase the total number of known agromyzid species in Spain to 287. In addition, M. spinulosa is a new report for Europe. Information on host plants, phenology, development, and distribution are included.