Cilia-associated respiratory bacillus-like organisms (CLO) are bacteria found intermixed among the cilia of epithelial cells lining the respiratory tract. They are considered moderate pathogens and have been reported in rodents, rabbits, ruminants, cats, and pigs. In this article, we describe respiratory disease associated with a Mycobacterium sp. and CLO in a banded rock rattlesnake (Crotalus lepidus klauberi). The CLO were confirmed via Warthin-Starry silver stain and ultrastructure examination.