Blood samples were collected from 18 female and 20 male adult crested geckos from 1 captive population in Massachusetts, USA. The geckos were given a thorough examination, and a blood sample was collected for a complete blood count (CBC) and plasma biochemistry analysis. Whole blood was stored in lithium heparin for analysis. The median weight of the geckos was 43.4 g (10–90%: 38.0–55.3; min–max: 32.0–69.0). The range of the packed cell volume (PCV) for the geckos was 23.0–45.0%. The lymphocyte was the most common leukocyte found on the CBC. The following parameters were found to be significantly different by sex: calcium (P = 0.0001), phosphorus (P = 0.0001), total protein (P = 0.03), albumin (P = 0.03), and PCV (P = 0.001). Overall, the geckos in this study were clinically healthy, and the ranges established from this population may be used as a basis for comparison in clinical cases.