A 5-yr-old, male Brazilian rainbow boa (Epicrates cenchria cenchria) presented with a partial spectacle avulsion that progressed to full spectacle avulsion following ecdysis. A porcine small intestinal submucosal graft was sutured over the cornea to protect the globe and allow the spectacle to regenerate. Treatment included topical antibiotic and lubricant ophthalmic medications. The porcine small intestinal submucosal graft was shed during ecdysis 30 days after placement, revealing peripheral regeneration of the spectacle with a 1- to 2-mm crust covering a presumed central defect. At the next ecdysis 39 days later, the spectacle had completely regenerated. Application of a porcine small intestinal submucosal graft in combination with topical antibiotic and lubricant ophthalmic medications for treatment of complete spectacle avulsion was successful in allowing spectacle regeneration and was well tolerated by the rainbow boa in this case.