The Burmese roofed turtle (Batagur trivittata), endemic to Myanmar, is a critically endangered species. Little is known about their physiology and health. Therefore, establishing physiologic reference intervals is essential for the implementation of conservation plans. This study establishes hematology and biochemistry reference intervals of a group of 23 captive Burmese roofed turtles. The animals were sampled during a 4 month period, outside the breeding season. Most blood values did not differ significantly based on animal sex. However, in females, lymphocyte count and concentrations of glucose and calcium were relatively higher, and potassium was lower compared to that of males. Better understanding of blood analytes will help strengthen ex situ management and healthcare protocols as well as improve ongoing conservation efforts for this important species.