Descriptions of the advertisement call are provided for three species of arthroleptid frogs from Malawi. The call of Arthroleptis reichei consists of the irregular repetition of one single whistling note with a frequency range of 1,600–4,000 Hz and a dominant frequency of 3,400–3,500 Hz. Although pulses are not clearly recognizable, notes show an evident subunit structure. The call of A. stenodactylus consists of the regular repetition of one single unpulsed whistling note with a frequency range from 2,700–4,900 and a dominant frequency of 3,200–3,400 Hz. The call of A. xenodactyloides consists of the regular and virtually endless repetition of one single clearly pulsed note with a frequency range of 4,900–7,500 and a dominant frequency of 6,300–6,500 Hz. On the basis of the available published data, two different call patterns are preliminarily recognizable in south and eastern African species belonging to Arthroleptis: (1) the repetition of one single unpulsed whistling-like note at low-medium (1,600–4,900 Hz) frequencies; and (2) the repetition of single pulsed cricket-like note at medium-high (4,600–7,500 Hz) frequencies.