We describe a new species of Pristimantis from the Amazonian lowlands in southern Peru (Madre de Dios Region). The new species has a snout–vent length of 22.8–23.4 mm in two adult males (females are unknown), a tympanum barely visible, a W-shaped scapular ridge, the iris bearing a dark vertical bar forming a cross or a T, a cream venter with brown blotches, and groin and concealed surfaces of shanks with a contrasting pattern consisting of yellow and black. It is tentatively assigned to the unistrigatus species Group and is most similar to Pristimantis diadematus and Pristimantis eurydactylus.