The Neotropical genus Pseudopaludicola includes 24 species distributed throughout South America. Herein, we formally describe the 25th member of this Neotropical clade as a new species: Pseudopaludicola jazmynmcdonaldae sp. nov. from an Amazon-Cerrado transitional zone in Brazil. The recognition of this new species is supported by adult morphology, advertisement call, and molecular data. It is diagnosed mainly by the following characters: small size; upper eyelids smooth, with no palpebral tubercles; heel smooth, with no enlarged conical tubercle; terminal phalanges knobbed, with no T-shaped terminal phalanges or expanded toe tips; relatively short hind limbs (tibiotarsal articulation just reaching the corner of the mouth); single, subgular, smooth, dark vocal sac in life, with no warty texture; and an advertisement call composed of regular series of short multipulsed notes, emitted at high rate.