Translator Disclaimer
1 July 2004 Syspastospora parasitica, a mycoparasite of the fungus Beauveria bassiana attacking the Colorado potato beetle Leptinotarsa decemlineata: A tritrophic association.
Francisco Posada, Fernando E. Vega, Stephen A. Rehner, Meredith Blackwell, Donald Weber, Sung-Oui Suh, Richard A. Humber
Author Affiliations +
Abstract

A tritrophic association is reported, involving a Colorado potato beetle (Leptinotarsa decemlineata) infected with Beauveria bassiana, which in turn was infected with Syspastospora parasitica.

Results

As part of a program for sampling Beauveria bassiana infections in the Colorado potato beetle (Leptinotarsa decemlineata (Say); Coleoptera: Chrysomelidae) collected from a potato field at the Beltsville Agricultural Research Center in Beltsville, Maryland, USA, we observed that 1 out of ca. 300 infected beetles collected on July 25, 2003, at a site known as Sleepy Hollow (N 39° 01.978′, W 76° 55.857′; 39 m above sea level), exhibited mycoparasitic growth on B. bassiana (Figure 1a–c). The mycoparasite was identified as Syspastospora parasitica (Tulasne) Cannon & Hawksworth.

The genus Syspastospora (Ascomycota: Sordariales) was erected by Cannon and Hawksworth (1982) to accommodate Melanospora parasitica, one of several species distinguished from species of Melanospora by characters that include a long perithecial neck composed of parallel hyphae and distinctive ascospores. In addition to S. parasitica two other species have been described: S. boninensis (Horie et al. 1986) and S. tropicalis (García et al. 2002), both isolated from soil. S. parasitica is known only as a mycoparasitic hyperparasite infecting various entomopathogenic clavicipitaceous fungi, e.g., Beauveria, Hirsutella, Paecilomyces, and some verticillioid species (Cannon and Hawksworth 1982). S. parasitica produces black perithecia with a small globose base and exaggeratedly long necks out of which copious quantities of cylindrical ascospores with markedly truncate ends are released (Fig. 1).

S. parasitica has been reported on various entomopathogenic fungi infecting insects, e.g., B. bassiana attacking the ash weevil Stereonychus fraxini (Coleoptera: Curculionidae) (Markova 1991); Cephalosporium spp. (= Verticillium) attacking the Kenya mealybug Planococcus kenya Le Pelley (Hemiptera: Pseudococcidae) (Masaba 1988); Paecilomyces farinosus attacking an unknown insect (ARSEF 5375; USDA-ARS Collection of Entomopathogenic Fungal Cultures, Ithaca, NY); Paecilomyces tenuipes attacking Bombyx mori (Lee and Nam 2000); B. bassiana infecting Cydia pomonella (L.) (Lepidoptera: Tortricidae); Beauveria tenella infecting Melolontha spp. (Coleoptera: Scarabaeidae); Spicaria farinosa (=Paecilomyces farinosus) infecting Boarmia bistortata Goeze (Lepidoptera: Geometridae); and Spicaria fumosorosea (= P. fumosoroseus) infecting Thaumetopoea pityocampa (Schiff.) (Lepidoptera: Thaumetopoeidae) (Müller-Kögler 1961). Ours is the first report of S. parasitica (ARSEF 7285) on a Colorado potato beetle infected with B. bassiana.

In our experience S. parasitica may go unobserved on the host. On two occasions when B. bassiana was transferred to potato dextrose agar from the Colorado potato beetle corpse, the presence of S. parasitica infection was not observed, although S. parasitica perithecia developed on the B. bassiana mycelial mat in approximately 60 days. Similar examples from inoculation of the coffee berry borer, Hypothenemus hampei (Ferrari) (Coleoptera: Curculionidae: Scolytinae), simultaneously with B. bassiana and Syspastospora ascospores resulted in B. bassiana-induced coffee berry borer mortality in around five days; it was not until three months later that S. parasitica perithecia appeared and produced ascospores. The slow development of S. parasitica perithecia also occurred when cultures of Syspastospora were started with ascospores that were contaminated with B. bassiana. In this case, B. bassiana grew within days but S. parasitica perithecia did not appear until almost one month later. In contrast, perithecia plated directly over B. bassiana cultures developed new ones in about 4 days at 25°C.

Acknowledgments

We thank Ann Sidor for collecting the insects. Part of this study was supported by the National Science Foundation (DEB-0072741 to MB).

References

1.

P. F. Cannon and D. L. Hawksworth . 1982. A re-evaluation of Melanospora Corda and similar Pyrenomycetes, with a revision of the British species. Botanical Journal of the Linnean Society 84:115–160. Google Scholar

2.

D. García, A. M. Stchigel, and J. Guarro . 2002. A new species of Syspastospora from tropical soils. Mycologia 94:862–865. Google Scholar

3.

Y. Horie, S. I. Udagawa, and P. F. Cannon . 1986. Four new species of the Ceratostomataceae (Ascomycetes). Mycotaxon 25:229–245. Google Scholar

4.

S. Lee and S-H. Nam . 2000. A mycoparasite Ascomycete Syspastospora parasitica on the entomopathogenic fungus Paecilomyces tenuipes growing in Bombyx mori. Mycobiology 28:130–132. Google Scholar

5.

G. Markova 1991. Melanospora parasitica attacking Beauveria bassiana on ash weevil Stereonychus fraxini in Bulgaria. Journal of Invertebrate Pathology 58:450–452. Google Scholar

6.

D. M. Masaba 1998. Coffee Physiology and Breeding. Review of Kenyan Agricultural Research Vol. 25:53. pp. Google Scholar

7.

E. Müller-Kögler 1961. Melanospora parasitica Tul. als Parasit der insektenpathogenen Beauveria tenella (Delacr.) Siem. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 68:600–605. Google Scholar

Figure 1.

(A) A tritrophic association with a Colorado potato beetle infected with Beauveria bassiana, which in turn is infected with Syspastospora parasitica; (B) close-up of S. parasitica perithecia; (C) perithecia growing on B. bassiana culture; (D) dissected perithecium with released ascospores; (E) ascospores moving to the tip of the perithecium; (F) close up of ascospores.

i1536-2442-4-24-1-f01.jpg
Francisco Posada, Fernando E. Vega, Stephen A. Rehner, Meredith Blackwell, Donald Weber, Sung-Oui Suh, and Richard A. Humber "Syspastospora parasitica, a mycoparasite of the fungus Beauveria bassiana attacking the Colorado potato beetle Leptinotarsa decemlineata: A tritrophic association.," Journal of Insect Science 4(24), 1-3, (1 July 2004). https://doi.org/10.1673/031.004.2401
Received: 9 March 2004; Accepted: 1 May 2004; Published: 1 July 2004
JOURNAL ARTICLE
3 PAGES


SHARE
ARTICLE IMPACT
Back to Top