Sirtuins, a family of NAD -dependent protein deacetylases, regulate important physiological events including aging and cell metabolism, mainly by protecting cells/tissues from oxidative damage. Ovarian aging decreases the quality of oocytes through induction of mitochondrial dysfunction and increases in DNA strand breaks by accumulation of reactive oxygen species. However, involvement of sirtuins in regulating oocyte quality with aging has not been determined. Here, we found the expression of sirtuin genes (Sirt1–7) in mouse ovaries and isolated oocytes and cumulus cells in a cell-specific manner. Based on real-time RT-PCR, all seven sirtuin genes were detected in the ovary with Sirt2 transcript levels showing the highest abundance. Oocyte expressed high levels of Sirt6, whereas the expressions of Sirt1, Sirt2, Sirt4, and Sirt6 were high in cumulus cells. When comparing samples from young and aged mice, oocyte levels of Sirt1–7 mRNA were not different. However, Sirt2 and Sirt6 transcript levels were decreased in cumulus cells of aged mice. Our findings suggest a possible association of Sirt2 and Sirt6 transcript levels in cumulus cells with impaired oocyte quality in aged mice. Further understanding the roles of these sirtuins in cumulus cell and oocyte could provide a better strategy to minimize aging-related decline in oocyte quality.