Assisted reproductive technology (ART) has greatly benefited numerous infertile couples who would never have had their babies without this technique. However, in vitro culture is reported to cause epigenetic and transcriptomic changes on preimplantation embryos, leading to adverse effect on development, and little is known about the molecular mechanisms underlying these changes. Here, we first introduce key studies that designate the effectiveness of an omic strategy to explore the molecular mechanisms governing preimplantation development of in vitro-cultured embryos. Furthermore, we review how in vitro culture components facilitate genomic reprogramming and zygotic genome activation (ZGA) contributing to preimplantation development after somatic cell nuclear transfer (SCNT). From these different perspectives, we would search for a breakthrough to further optimize preimplantation embryo culture conditions and improve ART.
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1 April 2016
Towards Further Optimization of Preimplantation Embryo Culture Media: from the Viewpoint of Omics and Somatic Cell Nuclear Transfer (SCNT) Studies
Mitsutoshi Yamada,
Toshio Hamatani,
Hidenori Akutsu,
Mamoru Tanaka
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Journal of Mammalian Ova Research
Vol. 33 • No. 1
May 2016
Vol. 33 • No. 1
May 2016
metabolomics
Octanoate
preimplantation embryo
SCNT
ZGA