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1 April 2017 Recent Technical Breakthroughs for ARTs in Mice
Keiji Mochida, Ayumi Hasegawa, Atsuo Ogura
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Abstract

Assisted reproductive technologies (ARTs) in mice were recently advanced when two long-existing technical barriers were overcome. The first barrier was the limited number of mature oocytes after conventional superovulation, especially in inbred strains of mice. A combination of estrous cycle synchronization and antiinhibin serum treatments increased the number of collected oocytes from female mice by approximately 3–4 times in many strains. The second barrier was the low fertilization rate after in vitro fertilization (IVF) using frozen-thawed spermatozoa. The addition of reduced glutathione in the fertilization medium dramatically increased the IVF yields, even in cryopreserved/warmed spermatozoa from the C57BL/6J strain, which is one of the strains most sensitive to cryoinjury. This result encouraged the use of cryopreserved spermatozoa in mouse strains worldwide for the preservation and transportation of their genetic characteristics. The final yield to produce offspring from one female was increased from 9 to 30. In IVF with cryopreserved spermatozoa from the C57BL/6J strain, the final yield using these technological innovations was estimated to be ninefold higher than previously. Following this improvement, the efficiency of ARTs in mice was increased dramatically and the decrease in the number of euthanized animals contributes to animal welfare and reduces labor and expense.

©2017 Japan Society for Ova Research
Keiji Mochida, Ayumi Hasegawa, and Atsuo Ogura "Recent Technical Breakthroughs for ARTs in Mice," Journal of Mammalian Ova Research 34(1), 13-21, (1 April 2017). https://doi.org/10.1274/032.034.0104
Received: 28 November 2016; Accepted: 1 January 2017; Published: 1 April 2017
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KEYWORDS
art
Frozen Sperm
IVF
Mouse
superovulation
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