The manner in which space is utilized by animals is influenced by several factors, including habitat quality and the distribution and abundance of resources. We used 4 years (2000–2003) of radiotelemetry data to investigate the space-use pattern of female Florida black bears (Ursus americanus floridanus) in the Ocala National Forest and an adjacent residential community of Lynne, north-central Florida. Annual home-range size (95% fixed kernel density estimator) ranged from 3.8 km² to 126.9 km², and averaged (± SE) 24.2 ± 3.55 km². Home ranges were largest during 2000 when a drought led to a forest-wide mast failure, suggesting that abundance of food resources can substantially influence space-use pattern. Home-range sizes during autumn (19.92 ± 4.59 km²) were substantially larger than during summer (8.26 ± 0.99 km²). Although annual home-range size did not differ between the 2 study sites, home ranges in summer were smaller in Lynne (5.30 ± 1.01 km²) than in Ocala National Forest (9.82 ± 1.29 km²), whereas home ranges in autumn were twice as large in Lynne (35.76 ± 13.91 km²) as in Ocala National Forest (13.24 ± 1.80 km²). We suggest that site-specific differences in the size of seasonal home range are due to differences in habitat characteristics and the degree of habitat fragmentation between the 2 study sites.