Accounting for low and heterogeneous detection probabilities in large mammal capture–recapture sampling designs is a persistent challenge. Our objective was to improve understanding of ecological and biological factors driving detection using multiple data sources from an American black bear (Ursus americanus) DNA hair trap study in south-central Missouri. We used Global Positioning System telemetry and remote camera data to examine how a bear's distance to traps, probability of space use, sex-specific behavior, and temporal sampling frame affect detection probability and number of hair samples collected at hair traps. Regression analysis suggested that bear distance to nearest hair trap was the best predictor of detection probability and indicated that detection probability at encounter was 0.15 and declined to < 0.05 at nearest distances > 330 m from hair traps. From remote camera data, number of hair samples increased with number of visits, but the proportion of hair samples from known visits declined 39% from early June to early August. Bears appeared attracted to lured hair traps from close distances and we recommend a hair trap density of 1 trap/2.6 km² with spatial coverage that encompasses potentially large male home ranges. We recommend sampling during the late spring and early summer molting period to increase hair deposition rates.