Monoterpenes are the main components of essential oils. Some members of this chemical family present insecticidal activity. Triatoma infestans (Klug) is the main vector of Chagas disease in Argentina, Bolivia, Paraguay, and Perú. The objective of this work was to evaluate the effect of six monoterpenes (1,8-cineole, eugenol, linalool, menthol, α-terpineol, and thymol) on the locomotor and flushing out activity of T. infestans. A video tracking technique was used to evaluate the locomotor activity of nymphs exposed to different concentrations of these chemicals applied as films on filter paper. Papers treated with acetone alone were used as negative controls, while solutions of tetramethrin were applied as positive controls. Only linalool and menthol produced hyperactivation. Flushing out was assessed under laboratory conditions using a standardized aerosolization method. All monoterpenes were applied at 1.5 g/m³. 1,8-Cineole, α-terpineol, and thymol flushed out 10% or less nymphs. The average flushing out produced by eugenol was 36.7%. Values of median flushing out time (FT₅₀) could only be calculated for linalool and menthol (16.67 and 42.98 min, respectively). The FT₅₀ value for the positive control tetramethrin (applied at 0.006 g/m³) was 8.29 min. Following these results, the flushing out activity of a mixture of linalool and eugenol was evaluated. The FT₅₀ of this 2:1 linalool:eugenol mixture was 40.73 min. Finally, flushing out assays performed in semifield conditions showed similar results to those obtained at the laboratory.