The present study evaluated the diversity of Anopheles Meigen, 1818 belonging to the subgenus Nyssorhynchus Blanchard, 1902 in four areas of Cachoeiras de Macacu municipality, Rio de Janeiro, Brazil.The immature collections were carried out during a rainy period and a post-rainy season. During the two periods, 3,932 specimens of Anopheles (Nyssorhynchus) were collected: 562 in the rainy period (14.29%) and 3,370 in the post-rainy period (85.70%). Among 3,932 specimens collected during both periods, 489 were identified, 50 (8.89%) in the rainy period and 439 (13.02%) in the post-rainy period. Nine Anopheles species were recorded: Anopheles albitarsis Lynch Arribálzaga, 1878 s.l. (76.86%), An. braziliensis (Chagas, 1907) (17%); An. evansae (Bréthes, 1926) (8, 48%); An. triannulatus (Neiva & Pinto, 1922) s.l. (8.23%); An. oswaldoi (Peryassú, 1922) (6.43%); An. strodei Root, 1926 (5.40%); An. argyritarsis Robineau-Desvoidy, 1827 (1.54%); An. rangeli Gabaldón et al., 1940 (1.28%); and An. aquasalis Curry, 1932 (0.51%). Among them, An. albitarsis was dominant and abundant in 93.75% of the collection points. Our results show a diverse Anopheles fauna in the municipality of Cachoeiras de Macacu, with a strong association of occurrence between An. rangeli and An. oswaldoi; An. rangeli and An. evansae; An. evansae and An. oswaldoi; and An. albitarsis s.l. and An. braziliensis during the rainy period. However, there was a weak correlation in the post-rainy period, except between An. rangeli and An. oswaldoi and between An. triannulatus and An. aquasalis, which displayed a moderate linear correlation. Our results suggest that following a potential reintroduction of malaria Cachoeiras de Macacu, local transmission of Plasmodium Marchiafava & Celli, 1885 may be enhanced by the presence of nine Nyssorhynchus species (all potential malaria vectors).