The dipteran fauna from the Brazilian Amazon remains poorly known, including that of forensic interest, such as the Sepsidae. The sepsid fauna of two secondary forests in the eastern Amazon was surveyed utilizing rotting bovine lung and feces (humans, bovines, and monkeys).We obtained 17 sespid species in six genera, most of them on bovine dung and rotting bovine lung. Two new species are described herein: Archisepsis bosque sp. nov. and Archisepsis verae sp. nov. The genus Palaeosepsis Duda, 1926 and the species Archisepsis polychaeta (Ozerov, 1993), Archisepsis diversiformis (Ozerov, 1993), Microsepsis mitis (Curran, 1927), Meropliosepsis sexsetosa Duda, 1926, and Palaeosepsis cf. golovastik Ozerov, 2004 are newly recorded from the Brazilian Amazon.