Translator Disclaimer
1 July 2010 Cave Crickets and Cave Weta (Orthoptera, Rhaphidophoridae) from the Southern End of the World: A Molecular Phylogeny Test of Biogeographical Hypotheses
Author Affiliations +
Abstract

In this study we reconstructed the molecular phylogeny and attempted to infer historical biogeography of a sample of cricket species, most of them cave-dwelling, belonging to the subfamily Macropathinae (Orthoptera, Rhaphidophoridae) which shows a clear Gondwanan distribution. We sequenced fragments of 4 genes (12S rRNA, 16S rRNA, 18S rRNA and 28S rRNA), for a total of 1993 bp.

We present here preliminary data based on a total of 17 species, 11 belonging to Macropathinae and representative of the main regions of Gondawanaland, 7 to Aemodogryllinae and Rhaphidophorinae from India, Bhutan, China, Philippines and the Sulawesi Islands.

The use of relaxed molecular clocks by means of Bayesian analysis allowed us to estimate the timing of the main cladogenetic events, using calibration of a molecular clock; the clock is based on the plate disjunction of Africa from South America, Australia from Zealandia (New Zealand), or Australia from Antarctica. The latter was considered at two different datings on the basis of two alternative palaeogeographic hypotheses. Node dating using separation of Africa or a model of earlier separation of Australia from Antarctica, suggests that the main cladogenetic events in the Macropathinae phylogeny could be explained by vicariance hypotheses, related to the Gondwana fragmentation. However, two other equally valid calibrations suggest that lineage formation is not consistent with vicariant processes and requires either some long-distance dispersal, or an inconceivable age of origin of this family of insects, enabling the prior existence of all lineages in Gondwanaland with subsequent regional extinction.

Introduction

Is plate tectonic vicariance a convincing hypothesis for the distribution of a group of palaeoantarctic Orthoptera Grylloacridoidea? The Orthoptera appear to have originated in the Carboniferous (360–300 Mya,) (Ragge 1955, Brauckmann et al. 1994), prior to the breakup of Pangea and Gondwana. Within the Orthoptera, taxa belonging to the family Rhaphidophoridae are considered as phylogenetically old by virtue of their morphology and their wide, disjunct geographic distribution across the temperate areas of the Boreal and Austral hemispheres (Hubbell & Norton 1978).

Various estimates of the time of origin of the Rhaphidophoridae have been proposed, including Upper Carboniferous (Ragge 1955), Permian (Ander 1939), Triassic (Beier 1955) and Jurassic (Sharov 1971), depending on the hypothesized sequence of phyletic branching and the known ages of the fossil Ensifera considered to be their closest relatives. Hubbell & Norton (1978) suggested that the origin of Rhaphidophoridae could not be more recent than the early Jurassic, since by the end of that epoch the separation of Gondwana had started and dispersal routes were broken.

This and other such inferences are heavily reliant on the a priori assumption that taxonomic diversity is explained by vicariance. However, there are few independent data to support this assumption. For instance, the only fossil record of the Rhaphidophoridae consists of three species of Prorhaphidophora described from Oligocene (35–25 Mya) Baltic amber (Chopard 1936), thus uninformative on the question.

Karny (1934) and Ander (1939) suggested that, on the basis of their current distribution, Rhaphidophoridae originated in the temperate areas of the southern hemisphere and spread northwards. In one scenario, an ancestral stock reached Laurasia and was the progenitor of all the northern hemisphere subfamilies.

All Rhaphidophoridae species are nocturnal and wingless, with a preference for humid habitats. Most are restricted to caves and appear to disperse little between these patchy habitats, and this lends support to the idea that they would have little potential for dispersal over sea.

At present, Rhaphidophoridae include nine subfamilies mainly distributed in the temperate regions of the northern and southern hemispheres and in the southeast Asia tropics. Among these, the Macropathinae are considered to be the sister group to all others in the family and have been referred to as “primitive” (Hubbell & Norton 1978). This subfamily comprises numerous species, belonging to about 30 genera, that together have a Gondwanan distribution in southwestern Australia, Tasmania, New Zealand and the surrounding islands, South America (Patagonia and Falkland Islands) and southern Africa (Cape Peninsula and the southern tip of South Africa), but not India, where the Rhaphidophoridae are represented by other subfamilies.

The breakup of Gondwana provides one of the most obvious test cases for vicariance biogeography. Gondwanaland started to break up around 130 Mya in early Cretaceous time into four continental blocks (South America, Africa, India-Madagascar and Australia-Antarctica-Zealandia(Veevers et al. 1980, Lawver et al. 1992, Li & Powell 2001, Trewick et al. 2007, Neall & Trewick 2008). India separated from Madagascar in the late Cretaceous (88–84 Mya) and began moving northward to collide with Asia at about 50 Mya. South America and Africa started to separate in the Early Cretaceous (130 Mya, Powell et al. 1988, Lawver et al. 1992) with the opening of the South Atlantic Ocean. The complete separation of Africa from South America happened during the Mid-Cretaceous (96 Mya; Powell et al. 1988, Lawver et al. 1992).Zealandia (later New Zealand), Australia, South America, and the then warm-temperate Antarctica, remained connected until late Cretaceous time: east Antarctica was closest to southern Australia and Zealandia, and southern South America was in contact with west Antarctica. Zealandia was the first to separate from Australia-Antarctica in the late Cretaceous (∼83 Mya) opening the Tasman Sea (Trewick et al. 2007, Campbell & Hutching 2007, Graham 2008). Zealandia subsequently thinned and submerged, with marine transgression peaking in late Oligocene time, and it is uncertain how much land, if any, persisted in the vicinity of New Zealand (Trewick et al. 2007, Landis et al. 2008). New Zealand began to form in early Miocene time with the abrupt onset of plate tectonic activity, but it is possible that one or more small islands existed prior to this (McLoughlin 2001). Australia and South America remained in contact via Antarctica until final separation and formation of the Southern ocean during the late Eocene (35 Mya). Although Australia and Antarctica started to separate in the late Cretaceous (90 Mya), the process is thought to have been completed in the late Eocene (∼35 Mya) (Veevers 1984, Exon et al. 2004). An alternative proposal for the earlier existence of a shallow marine seaway between Australia and Antarctica suggests separation by 50 Mya (Woodburne & Case 1996). Southern South America and Antarctica remained in contact through the Antarctic Peninsula until the Oligocene (35–28 Mya), when the Drake Passage opened between these continents (Lawver et al. 1992, Dingle & Lavelle 2000).

Table 1.

Raphidophoridae species and outgroup taxa included in this study.

t01a_121.gif

Continued.

t01b_121.gif

Meta-analyses of area cladograms (Sanmartin & Ronquist 2004) show that many animal phylogenies match the sequential breakup of Gondwana and are thus consistent with a plate tectonic vicariance hypothesis. However, there is an increasing number of southern hemisphere taxa for which divergence time estimates do not fit the temporal framework of fragmentation (Cooper et al. 2001, Van Bocxlaer et al. 2006, Raxworthy et al. 2002) leading to the conclusion that the distribution of these organisms resulted from overseas dispersal events (Raxworthy et al. 2002). In this paper we examined the historical biogeography of Macropathinae, using taxa representative of each of the regions encompassed by the subfamily. We also considered species belonging to two Asian subfamilies that are the putative sister groups to Macropathinae: Aemodogryllinae and Rhaphidophorinae from India, Bhutan, China, Philippines and the Sulawesi Islands. From the biogeographical point of view, these insects, being apterous and characterized by low vagility, could be responsive to vicariance biogeography.

We present analysis of preliminary mitochondrial and nuclear DNA data using a relaxed molecular clock approach with Bayesian analysis. The molecular clock was calibrated using the dated breakup of Gondwanaland. We considered one palaeogeographic event at time, and then assessed the extent of support for vicariance implied across the phylogeny. In this way, alternative biogeographic hypotheses (based on the available palaeogeographic data available for a given region) were analysed to determine the most plausible scenario that may explain the current distributional pattern of Macropathinae. We apply the null hypothesis that lineage formation (phylogenetic splitting) resulted from sundering of populations by plate tectonic vicariance, and ask whether: 1 ) within the constraints of the available sampling, is Macropathinae tree topology consistent with the timing of Gondwanan breakup? 2) Are estimates of the age of inferred ancestors consistent with this process? 3) Do different calibrations result in agreement or do they each require a unique biogeographic hypothesis to explain the Gondwanan influence on the distribution of Macropathinae lineages?

Materials and Methods

Taxon sampling.—In this study a total of 17 species were assayed (Table 1). In particular, we considered 11 species among Macropathinae: Spelaeiacris tabulae and Spelaeiacris monslamiensis from South Africa; Heteromallus cavicola, Udenus w-nigrum and Parudenus flaklandicus from South America; Micropathus tasmaniensis from Tasmania; Pachyramma edwardsii, Pleioplectron simplex, Tallitropsis sedilotti, Pallidoplectron turnerii and Macropathus filifer from New Zealand.

Among Aemodogryllinae, we sampled species belonging to genera Atachycines, Diestrammena and Diestramima from India, China and Bhutan and among Rhaphidophorinae, species belonging to genus Raphidophora from the Philippines and Sulawesi Islands. As an outgroup we used representatives of other subfamilies: Nearctic Ceuthophilinae (Hadenoecus cumberlandicus, Euhadenoecus insolitus) and Palaearctic Troglophilinae (Troglophilus cavicola) and Dolichopodainae (Dolichopoda sbordonii). We analyzed more than one individual for each species where samples were available (Table 1). Our sampling included recently collected specimens as well as those from museum collections.

Laboratory procedures.—DNA was isolated from leg muscles from specimens preserved in 85–95% EtOH, following either a C-TAB extraction protocol (Doyle & Doyle 1987) or a salting-out method (Cook et al. in press).

Four gene fragments from each DNA sample were amplified using the polymerase chain reaction (PCR), corresponding to the genes 12S, 16S, 18S and 28S rRNA subunits. PCR and sequencing of 28S rRNA targeted domains D1 and D3–D5. Age and often suboptimal preservation of some museum samples meant that DNA was highly fragmented. So it was necessary to use reamplification with a set of nested primers to obtain complete sequences.

Initial PCR reactions used the primers 485 and 689, and 427 and 686 for amplification of the D3–D5 and D1 domains of the 28S rDNA gene (Friedrich & Tautz 1997); 8F and 9R for 18S (Giribet et al. 1996); 12SAi and 12SBi for 12S (Simon et al. 1994) and 16SAr and 16SBr for 16S rRNA (Palumbi 1996). Internal primers used in the nested-PCRs were designed from those sequences that could be obtained with a single PCR reaction with the primers above (See Table 2). Double-stranded amplifications were performed with a Perkin-Elmer-Cetus thermal cycler (PE Applied Biosystems, Foster City, CA, USA) in 25 µl of a solution containing genomic DNA, 1.5 mM MgCl2, 2.5 mM of each dNTP, 0.5 µM Primer, 1 U EuroTaq (EuroClone) and the buffer supplied by the manufacturer.

Table 2.

Internal primers specifically designed for Rhaphidophoridae.

t02_121.gif

Standard PCR conditions included a 2-min denaturation at 94°C followed by 5 cycles at 95°C for 15s, 48°C for 45s, and 62°C for 40s, and 30 cycles at 95°C for 30s, 50°C for 30s, and 72°C for 30s, with a final extension at 72°C for 3min. The nested-PCRs included a 2-min denaturation at 94°C, followed by 30 cycles at 95°C for 30s, 50°C for 30s, and 72°C for 30s, with final extension at 72°C for 3 min. These parameters varied for each primer pair used, mainly using higher or lower annealing temperatures.

Double-stranded amplified products were checked for expected size by electrophoresis of 1/10 of the product through a 1% agarose gel. PCR fragments were purified using the ExoSAP digestion (GE Healthcare Europe, Munich, Germany), directly sequenced in both directions using the ‘Big Dye Terminator Ready-reaction Kit’ and resolved on ABI 3100 Genetic Analyzer (PE Applied Biosystems, Foster City, CA, LISA), following the manufacturer protocols. Sequence data were edited and compiled using Codon Code Aligner version 3.5.2 (Codon Code Corporation) or Sequencher ver. 4.2 sequence editor (Gene Codes Corp., Michigan).

Phylogenetic analysis.—Sequences of the four gene fragments, 12S, 16S, 18S, 28S (D1 and D3–D5 domains) were aligned separately using CLUSTALX 2.0 (Thompson et al. 1997) with opening gap = 10 and extended gap = 0.10. The alignments were also checked by eye. Models for DNA substitution were selected independently for each gene using MODELTEST 3.0.6 (Posada & Crandall 1998). Phylogenetic analyses were performed on the concatenated dataset, partitioned by genes. We used maximum-likelihood (ML, Felsenstein 1981), as implemented in TREEFINDER 2008 (Jobb 2004), and Bayesian estimation as implemented in MRBAYES 3.1.2 (Huelsenbeck & Ronquist 2001), using the model selected by MODELTEST. One thousand bootstrap replicates were carried out in ML analysis, while Bayesian analysis was performed using four search chains for 1,000,000 generations, sampling trees every 100 generations. The first 1,000 trees were discarded as burn-in. Parameter stability was estimated by plotting log-likelihood values against generation time, and a consensus tree with posterior probabilities was then generated.

Fig. 1.

Relationship between the number of transitions and/or transversions and genetic distance values (GTR distances).

f01_121.eps

Dating of the cladogenetic events.—A likelihood ratio test (LRT) was used to test the molecular clock hypothesis, according to Huelsenback & Crandall (1997). The LRT results suggested a heterogeneous rate variation for each gene and therefore a clocklike evolution of sequences could not be assumed. Consequently, dates of divergence were inferred using a relaxed molecular clock, following the uncorrelated relaxed lognormal clock (Drummond et al. 2006) as implemented in BEAST (v. 1.4.7, Drummond & Rambaut 2007). This analysis was performed on the concatenated matrix, partitioned by genes. A YuIe or “pure birth” prior process was applied to model speciation. The time to the most recent common ancestor (MRCA) between each clade was estimated under the model parameters highlighted in MODELTEST (Posada & Crandall 1998) for each gene. Two independent runs of 10 million steps were implemented with BEAST, with one million steps discarded as burn-in. Convergence to stationarity and effective sample size (ESS) of model parameters were assessed using TRACER 1.4 (Rambaut & Drummond 2003).

Current estimates of the timing of plate tectonic vicariance events were used to calibrate the molecular clock under the null hypothesis, that separation of land areas by tectonics was the cause of phylogenetic splitting in these taxa. Specifically, we considered separation of South Africa from Gondwana, separation of Australia from Zealandia and separation of Australia from Antarctica. When considering alternative palaeogeographic interpretations, these yield four potential calibrations: (i) isolation of South Africa 96–117 Ma (Powell et al. 1988, Lawver et al. 1992), (ii) the separation of Australia from Zealandia (New Zealand) 80–82 Ma (Li & Powell 2001), (iii) separation of Australia from Antarctica 35–40 Ma (Veevers 1984, Lawver et al. 1992) and (iv) separation of Australia from Antarctica 50–54 Ma (Woodburne & Case 1996). We calibrated the tree using one calibration point at a time, to assess the effect on inferred age of the other nodes, comparing this with geological age estimates.

Fig. 2.

Relationships among species of Rhaphidophoridae inferred by Bayesian analysis. The ingroup is indicated by the grey square. Values above the branches indicate posterior probabilities (PP) derived from Bayesian analysis.

f02_121.eps

Results

The total length of the alignment of the four gene fragments was 1993 bp, consisting of 414 bp 12SrRNA, 583 bp 16SrRNA, 173 bp 18SrRNA, and 813 bp 28SrRNA. The Ts/Tv ratio ranged from 0.17 to 5 for 12S, from 0 to 6 for 16S and 18S and from 0 to 8 for 28S. The Ts accounted for about 36% of all substitutions in mitochondrial genes and for about 63% in the nuclear ones, if outgroups are considered. Saturation was not observed, as demonstrated by the relationship between the number of Ts and Tv versus genetic distances (Fig. 1). MODELTEST indicated the general time-reversible model with among-site rate heterogeneity and a proportion of invariant sites for the mitochondrial genes and the nuclear 28S (GTR + I + Γ). GTR with the proportion of invariant sites was also indicated for 18S, but without among-site rate variation (GTR +I).

ML and Bayesian analyses gave trees of similar topology with the same nodes supported in all analyses (Fig. 2). Two principal clades can be distinguished, one including the Asian taxa and the other the palaeo-austral Macropathinae. The Asian clade consists of two clusters corresponding to the two subfamilies, Rhaphidophorinae and Aemodogryllinae. Among Macropathinae we can distinguish four well-supported clades, corresponding almost perfectly with South American, Tasmanian, New Zealand and South African taxa. The South African taxa were sister to the other Macropathinae sampled.

Fig. 3.

Divergence times among Rhaphidophoridae species inferred by Bayesian analysis using a relaxed molecular clock and different calibration points. A) Calibration point considering the isolation of Africa at 96 Mya; B) Calibration point considering the separation of Australia from Antarctica at 50 Mya; C) Calibration point considering the separation of Australia from Antarctica at 35 Mya; D) Calibration point considering the separation of Australia from New Zealand at 80–82 Mya. Bars at the nodes represent the 95% highest posterior density (HPD) credibility interval. Ingroup is indicated by grey box.

f03_121.eps

The data showed a nonclock-like behavior, with a coefficient of variation of 0.443 (95% highest posterior density, HPD: 0.222-0.688) if separation of Africa is considered. However, values for the coefficient of variation obtained when considering the other calibration points were very similar to one another. Using dated palaeoevents for the study region allowed us to calibrate the molecular clock and to estimate the divergence times of lineages enforcing a vicariance hypothesis. Four chronograms obtained using the different calibration points show many similarities, but are not identical in inferred node ages. Calibration using the separation of Africa from South America (96 Mya) indicates that divergence times between taxa in South America and Australasia range from 36 to 73 Mya (95% HPD) with a median of 55 Mya; between Australia and New Zealand taxa range from 31 to 66 Mya (95% HPD) with a median of 48 Mya (Fig. 3A). Calibration using the separation of Australia from Antarctica at 50 Mya resulted in estimates for isolation of the South African Macropathinae lineage between 62 and 110 Mya (95% HPD) with a median of 83 Mya, while the split between New Zealand and Australian taxa is dated at 42 Mya (95% HPD: 32.5–51 Mya) (Fig. 3B). Alternatively, applying a 35 Mya separation date of Australia and Antarctica results in the inferred isolation of South African taxa at 61 Mya (95% HPD: 44–81 Mya) and the divergence of Australia and New Zealand taxa at 31 Ma (95% HPD: 26 to 35 Mya) (Fig. 3C). When we applied a calibration based on the separation of Zealandia (New Zealand) from Australia at 82 Mya, we obtained estimates for isolation of South African Macropathinae at 155 Mya (95% HPD: 107–219 Mya), and divergence of South American and Australasian taxa at 91.5 Mya (95% HPD: 82–108 Mya; Fig. 3D). This calibration also returned an estimate for the basal node in our Rhaphidophoridae phylogeny of close to 600 Mya.

Molecular substitution rates per lineage per million years were also obtained for each analysed gene. These rates are broadly consistent with estimates previously obtained for insects (Brower 1994, Friedrich & Tautz 1997) and those obtained for Dolichopoda species (Allegrucci et al. unpub.), although all our inferred substitution rates are lower (Table 3). We found that calibration with 82 Mya for a Zealandia split from Australia/Antarctica, resulted in the lowest rates, least consistent with other estimates. Calibration using a 35 Mya date for the split of Antarctica and New Zealand, yielded inferred substitution rates closest to those previously published.

Table 3.

Substitution rates per gene / site / lineage / million years in Rhaphidophoridae species here analysed. Substitution rates are reported for each used calibration point.

t03_121.gif

Discussion

The analysis resulted in a well-resolved and generally strongly supported phylogeny. Taxa are, for the most part, clustered by geographic areas, with each main clade corresponding to a particular geographic region. Asian species are, in our analyses, sister to the palaeo-austral Macropathinae, and among the Macropathinae, the South African S.tabulae and S. monslamiensis are sister to the transPacific taxa. Africa, as the first continent to separate from Gondwana, could have led to isolation of this lineage, while exchange continued between Australia and South America via Antarctica.

Thus, in terms of geographic distribution, we can say that the Macropathinae phylogeny is consistent with a vicariant history, with one exception. In our analysis of the current taxon sample and data, we find that M. filifer does not group with other New Zealand Macropathinae (although we also note that this is an unresolved node). This is surprising, and not as predicted from a vicariant biogeographic hypothesis. However, a similar lack of monophyly among New Zealand Orthoptera has been reported for the Anostostomatidae, in which high lineage diversity contrasts with the relatively small land area and young geological history (Pratt et al. 2008) and demands further study. Among the Rhaphidophoridae in our present analysis, the Asian subfamilies (Aemodogryllinae, Rhaphidophorinae) are revealed as sister to the Macropathinae. This is also consistent with plate tectonic vicariance, as India separated from other parts of Gondwana at about 130 Mya, prior to other vicariance events.

Estimating rates of molecular evolution and consequent divergence times is a critical issue and its reliability depends on the suitability of the calibration method and the choice of calibration event(s). Here we applied a consistent approach, but varied calibration points to assess the consistency of inferred node ages. Assuming models of DNA evolution are appropriate, rate variation across the tree is accommodated, calibration points are correctly dated and assumptions about vicariant process are valid (or at least clearly stated, see null hypothesis), we can expect consistent estimates of node age among analyses if plate tectonic vicariance is the primary driver of lineage formation in this group. In the present case and following the complex geological history of Gondwanaland masses, we referred to four major geological events to calibrate our molecular clock.

We considered the separation of South America and Africa 96–117 Mya (Fig. 3A; Powell et al. 1988, Lawver et al. 1992), and it is immediately evident that the topology of our phylogeny with the first major split with the Macropathinae is consistent with this model. This analysis suggests that exchange between South America and Australasia (via Antarctica) was interrupted about 60 Mya, close to the time at which it is estimated that a shallow marine seaway formed between Antarctica and Australia (50 Mya, Woodburne & Case 1996). Inferred divergence of New Zealand and Australian Macropathinae between 31–66 Mya is younger than isolation of Zealandia from Australia. Calibration using the separation of Australia from Antarctica due to the formation of a shallow marine seaway between the two continents (50 Mya; Woodburne & Case 1996), returned divergence estimates congruent with the above, and geological estimates for isolation of Africa, but not separation of Zealandia and Australia (Fig. 3B).

Calibration using a younger, but widely cited, age for separation of Australia from Antarctica at 35 Mya yielded dates for other nodes that are too young for tectonic vicariance of taxa in other regions (Fig. 3C). Conversely, using an age of 82 Mya for the split of Zealandia (later New Zealand) from Australia/Antarctica, a date used in calibration of several bird phylogenies (see discussion in Trewick & Gibb 2010), gave inferred formation of lineage prior to tectonic events for isolation of S. America, S. Africa and Australia. Indeed, the root of the Rhapidophoridae was pushed back to nearly 600 million years (Fig. 3D).

In our calibrations the inferred age for the split of Indian (and other Asian) taxa from Macropathinae is near (Fig. 3C), or older than, the probable time of separation of India from Gondwana. This implies that either the Asian taxa are not (contrary to morphological evidence and unpublished molecular analyses) sister to Macropathinae, or the most appropriate calibration for the Macropathinae is the 35 Mya separation of Australian and S. America taxa (Fig. 3C), or that some process other than vicariance has been involved in the biogeography of the Rhaphidophoridae. We note that our other calibrations would put the root of the Rhaphidophoridae close to the hypothesized origination of the Orthoptera (i.e., Carboniferous; Fig. 3A, 3B) or prior to this (Fig. 3D). As India is estimated to have started docking with Asia about 50 Ma, this route into the Asian continent would not have been available earlier, and is younger than all estimates for the age of the Asian clade.

In conclusion, we find topological support or at least agreement with a plate-tectonic vicariance process for biogeography of Macropathinae, and its putative Asian sister group. Node dating using separation of Africa or a model of earlier separation of Australia from Antarctica, are roughly in agreement and yield dates for other nodes that are closest to geological ages.

However, two other, equally valid calibrations, yield dates that are not consistent with a vicariant process for lineage formation, and require, at least some long-distance dispersal, or prior existence of all lineages in Gondwana, with subsequent regional extinction, or an implausible age of origin of this family of insects.

When we consider inferred rates of molecular substitution as well, we are forced to concede that inclusion of long-distance dispersal events (LDD) would simply reconcile phylogeny, timing and geological history. This has been the case in several studies of palaeo-Austral animals and plants (e.g., birds — Trewick & Gibb 2010; plants — Meudt & Simpson 2006, Wagstaff & Hennion 2007, Knapp et al. 2005). However, the idea that LLD has occurred within this group is difficult to reconcile with much that is known about their ecology and population genetics (e.g., Sbordoni et al. 1985, 1987, 1991, 2000, 2005; Carchini et al. 1991; Cesaroni et al. 1997; Allegrucci et al. 1987, 1992, 1997, 2005, 2009).

European and North American (Caccone & Sbordoni 1987) taxa that have been studied tend to show strong partitioning of population genetic structure and species diversity among geographic areas (Sbordoni et al. 1985, Allegrucci et al. 1997), implying little exchange. However, the reconstruction of phylogeny in genus Dolichopoda and the testing of different geographic hypotheses (Allegrucci et al., 2005, 2009 and unpub.) would suggest that LDD could have played an important role before these crickets became isolated in caves by arid climates. Indeed, a strong phylogeographic pattern has been observed and the temporal sequence of cladogenetic events is in good agreement with the geographic distribution of Dolichopoda studied; this supports the inference that speciation events are strictly allopatric and mostly determined by isolation of different populations in isolated cave systems (Allegrucci et al. 2009 and unpub.).

Studies of some of the diverse New Zealand Rhaphidophoridae reveal more extensive gene flow among populations (Cook et al. 2010), and close relationships between species on different islands (Trewick 2000, Goldberg et al. 2008). Indeed, in the New Zealand region, several offshore islands have endemic rhaphidophorids. In New Zealand, Tasmania and Chile, Rhaphidophoridae live in temperate wet forests (SAT pers. obs), and are not restricted to cave habitats. In such environments, there is ample opportunity for movement across wide geographic scales, and in addition, occupation of holes in logs (rather than caves) is the best explanation for the colonization of islands (Trewick 2000).

Future work will use enhanced taxonomic sampling, habitat description, population genetics and phylogenetics to generate more sophisticated biogeographic and ecogeographic hypotheses to enhance our understanding of the evolutionary history of this group.

Acknowledgements

We are grateful to Mauro Rampini and Leonardo Latella who provided samples from China and to A. Hitchcock and P. Swart for providing samples from South Africa. Mauro Rampini helped us to identify samples from Asia. This research was financially supported by PRIN (the Italian Program for Relevant National Researches) grant to Valerio Sbordoni.

References

  1. G. Allegrucci , D. Cesaroni , V. Sbordoni 1987. Adaptation and speciation of Dolichopoda cave crickets (Orth. Rhaph.): geographic variation of morphometric indices and allozyme frequencies. Biological Journal of Linnaean Society 31: 151–160. Google Scholar

  2. G. Allegrucci , A. Caccone , D. Cesaroni , V. Sbordoni 1992. Evolutionary divergence in Dolichopoda cave crickets: a comparison of single copy DNA hybridization data with allozymes and morphometric distances. Journal of Evolutionary Biology 5: 121–148. Google Scholar

  3. G. Allegrucci , M.G. Minasi , V. Sbordoni 1997. Patterns of gene flow and genetic structure in cave-dwelling crickets of the Tuscan endemic, Dolichopoda schiavazzii (Orthoptera, Rhaphidophoridae). Heredity 78: 665–673. Google Scholar

  4. G. Allegrucci , V. Todisco , V. Sbordoni 2005. Molecular phylogeography of Dolichopoda cave crickets (Orthoptera, Rhaphidophoridae): a scenario suggested by mitochondrial DNA. Molecular Phylogenetics and Evolution 37: 153–164. Google Scholar

  5. G. Allegrucci , M. Rampini , P. Gratton , V. Todisco , V. Sbordoni 2009. Testing phylogenetic hypotheses for reconstructing the evolutionary history of Dolichopoda cave crickets in the eastern Mediterranean. Journal of Biogeography 36: 1785–1797. Google Scholar

  6. K. Ander 1939. Vergleichend-anatomische und phylogenetische Studien iiber die Ensifera (Saltatoria). Opuscula Entomologica, 306 pp. Google Scholar

  7. M. Beier 1955. Embioidea und Orthopteroidea. In: H. G. Bronn's Klassen und Ordnungen des Ticrreichs. Leipzig, Geest & Portig, Bd. 5, Abt. 111, Buch 6,304 pp. Google Scholar

  8. C. Brauckmann B. Braukmann , E. Gröning 1994. The stratigraphical position of the oldest known Pterygota (Insecta, Carboniferous, Namurian). Annales de la Société Géologique de Belgique 117: 47–56. Google Scholar

  9. A.V.Z. Brower 1994. Rapid morphological radiation and convergence among races of the butterfly Heliconius hereto inferred from patterns of mitochondrial DNA evolution. Proceedings National Academy of Sciences USA 91: 6491–6495. Google Scholar

  10. A. Caccone , V. Sbordoni , 1987. Molecular evolutionary divergence among North American cave crickets. I. Allozyme variation. Evolution 41: 1198–1214 Google Scholar

  11. G. Carchini , C. Di Russo , V. Sbordoni 1991. Contrasting age structures in cave cricket populations: patterns and significance. Ecological Entomology, 16: 305–314. Google Scholar

  12. H. J. Campbell , L. Hutching 2007. In Search of Ancient New Zealand. Auckland : Penguin and Lower Hutt, New Zealand: GNS Science. Google Scholar

  13. D. Cesaroni , P. Matarazzo , G. Allegrucci , V. Sbordoni 1997. Comparing patterns of geographic variation in cave crickets by combining geostatistic methods and Mantel tests. Journal of Biogeography 24: 419–431. Google Scholar

  14. L. Chopard , 1936. Biospeleologica N. LXIII. Orthopteres et Dermaptéres (Premiere serie). Archives de Zoologie Experimentale et Generale Paris 78: 195–214. Google Scholar

  15. L. Cook , S.A. Trewick , M. Morgani-Richards , P Johns 2010. Status of the New Zealand cave weta (Rhaphidophoridae) genera Pachyrhamma, Gymnoplectron and Turbottoplectron. Invertebrate Systematics 24: in press. Google Scholar

  16. A. Cooper , C. Lalueza-Fox , S. Anderson , A. Rambaut , J. Austin , R. Ward 2001. Complete mitochondrial genome sequences of two extinct moas clarify ratite evolution. Nature 409: 704–707. Google Scholar

  17. R.V. Dingle , M. Lavelle 2000. Antarctic Peninsula late Cretaceous - Early Cenozoic palaeoenvironments and Gondwana palaeogeographics. Journal of African Earth Sciences 31: 91–105. Google Scholar

  18. J.J. Doyle , J.L. Doyle 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistiy Bulletin 19: 11–15. Google Scholar

  19. A.J Drummond , S.H.W Ho , M.J Phillips , A. Rambaut 2006. Relaxed phylogenetics and dating with confidence. PLoS Biology 4: 699–710. Google Scholar

  20. A.J. Drummond , A. Rambaut 2007. BEAST v1.4.7. Bayesian Evolutionary Analysis Sampling Trees. Computer program. Available at  http://beast.bio.ed.ac.uk  Google Scholar

  21. N.F. Exon , J.P. Kennett , M.J. Malone 2004. Leg 189 synthesis: CretaceousHolocene history of the Tasmanian gateway. Proceedings of the Ocean Drilling Program, Scientific Results, 189. ISSN 1096–7451 doi: 10.2973/ odp.procsr.189.2004 Google Scholar

  22. J. Felsenstein 1981. Evolutionary trees from DNA sequences, a maximum likelihood approach. Journal of Molecular Evolution 17: 368–376. Google Scholar

  23. M. Friedrich , D. Tautz 1997. An episodic change of rDNA nucleotide substitution rate has occurred during the emergence of the insect order Diptera. Molecular Biology and Evolution 14: 644–653. Google Scholar

  24. G. Giribet , S. Carranza , J. Baguña , M. Riutort , C. Ribera 1996. First Molecular Evidence for the Existence of a Tardigrada + Arthropoda Clade. Molecular Biology and Evolution 13: 76–84. Google Scholar

  25. J. Goldberg , S.A. Trewick , A.M. Paterson 2008. Evolution of New Zealand's terrestrial fauna: a review of molecular evidence. Philosophical Transactions Royal Society of London B. Biological Sciences, 363: 3319–3334. Google Scholar

  26. I.J Graham , 2008. A Continent on the Move: New Zealand Geoscience into the 21st Century. Wellington: Geological Society of New Zealand & GNS Science. Google Scholar

  27. T.H. Hubbell , R.M. Norton 1978. The systematics and the biology of the cave-crickets of the North American tribe Hadenoecinni (Orthoptera Saltatoria: Rhaphidophoridae: Dolichopodinae). Miscellaneous Publications Museum of Zoology University of Michigan 156: 1–125. Google Scholar

  28. J. P. Huelsenbeck , K.A. Crandall 1997. Phylogeny estimation and hypothesis testing using maximum likelihood. Annual Review of Ecology and Systematics 28: 437–466. Google Scholar

  29. J.P Huelsenbeck , F. Ronquist 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 17: 754–755. Google Scholar

  30. G. Jobb , A. von Haeseler , K. Strimmer 2004. TREEFINDER: a powerful graphical analysis environment for molecular phylogenetics. BMC Evolutionary Biology 4: 18–27. Google Scholar

  31. I.H. Karny 1934. Zur Kenntnis der ostasiatischen Rhaphidophorinen (Orth., Salt., Gryllacrididae). Konowia 13: 70–80, 111–124, 214–230. Google Scholar

  32. M. Knapp , K. Stöckler , D. Havell , F. Delsuc , F. Sebastiani , P.J. Lockhart 2005 Relaxed molecular clock provides evidence for long-distance dispersal of Nothofagus (southern beech). PLoS Biology, 3: e14. Google Scholar

  33. C.A. Landis , H.J. Campbell , J.G. Begg D.C. Mildenhall , A.M. Paterson , S.A. Trewick 2008. The Waipounamu erosion surface: questioning the antiquity of the New Zealand land surface and terrestrial fauna and flora. Geological Magazine 145: 173–197. Google Scholar

  34. L.A. Lawver , L.M. Gahagan , M.F. Coffin , 1992. The development of paleoseaways around Antarctica. The Antarctic paleoenvironment: a perspective on global change. American Geophysical Union Antarctic Researches 56: 7–30. Google Scholar

  35. Z.X. Li , C.McA. Powell 2001. An outline of the palaeogeographic evolution of the Australasian region since the beginning of the Neoproterozoic. Earth-Science Reviews 53: 237–277. Google Scholar

  36. S. McLoughlin 2001. The breakup history of Gondwana and its impact on pre-Cenozoic floristic provincialism. Australian Journal of Botany 49: 271–300. Google Scholar

  37. H.M. Meudt , B.B. Simpson 2006. The biogeography of the austral, subalpine genus Ourisia (Plantaginaceae) based on molecular phylogenetic evidence: South American origin and dispersal to New Zealand and Tasmania. Biological Journal of Linnaean Society 87: 479–513. Google Scholar

  38. V.E Neall , S.A. Trewick 2008. The age and origin of the Pacific islands: a geological overview. Philosophical Transactions Royal Society of London B. Biological Sciences 363: 3293–3308. Google Scholar

  39. S.R. Palumbi 1996. Nucleic acids II: the polymerase chain reaction, pp 205–248. In: D.H. Hillis , C. Moritz , B.K. Mable (Eds), Molecular Systematics, Sinauer, Sunderland, MA. Google Scholar

  40. D. Posada , K. Crandall 1998. MODELTEST: testing the model of DNA substitutions. Bioinformatics 14: 817–818. Google Scholar

  41. C.McA. Powell , S.R. Roots , J.J. Veevers 1988. Pre-breakup continental extension in eastern Gondwanaland and the early opening of the eastern Indian Ocean. Tectonophysics 155: 261–283. Google Scholar

  42. R. Pratt , M. Morgan-Richards , S.A. Trewick 2008. Diversity within New Zealand weta (Orthoptera: Ensifera: Anostostomatidae) and their relationships in Australasia. Philosophical Transactions Royal Society of London B. Biological Sciences 363: 3427–3437. Google Scholar

  43. D.R. Ragge 1955. The Wing-venation of the Orthoptera Saltatoria. London, British Mus. (Nat. Hist.), 159 pp. Google Scholar

  44. A. Rambaut , A.J. Drummond 2003. TRACER (computer program) Available at:  http://evolve.zoo.ox.ac.uk/Evolve/Software.html  Google Scholar

  45. C.J. Raxworthy , M.R.J. Forstner , R.A. Nussbaum 2002. Chameleon radiation by oceanic dispersal. Nature 415: 784–787. Google Scholar

  46. I. Sanmartin , F. Ronquist 2004. Southern hemisphere biogeography inferred by event-based models: plant versus animal patterns. Systematic Biology 53: 216–243. Google Scholar

  47. V. Sbordoni , G. Allegrucci , D. Cesaroni , M. Cobolli Sbordoni , E. De Matthaeis 1985. Genetic structure of populations and species of Dolichopoda cave crickets: evidence of peripatric divergence. In: V. Sbordoni (Ed.), Genetics and Ecology in Contact Zones of Populations. Bollettino di Zoologia 52: 95–114. Google Scholar

  48. V. Sbordoni , G. Allegrucci , A. Caccone , G. Carchini , D. Cesaroni 1987. Microevolutionaiy studies in Dolichopodinae cave crickets, pp.514–540. In: B. Baccetti (Ed.) Evolutionary Biology of Orthopteroid Insects Horwood Ltd, Publ, Chichester, U.K. Google Scholar

  49. V. Sbordoni , G. Allegrucci , D. Cesaroni 1991. A multidimensional approach to the evolution and systematics of Dolichopoda cave crickets, p. 171–199. In: G.M. Hewitt et al. (Eds) Molecular techniques in taxonomy, NATO ASI Series, Vol. H 57. Springer Verlag, Berlin, Heidelberg. Google Scholar

  50. V. Sbordoni , G. Allegrucci , D. Cesaroni 2000. Population genetic structure, speciation and evolutionary rates in cave dwelling organisms, pp.453–477. In: H. Wilkens , D.C. Culver , W.F. Humphrey (Eds), Ecosystems of the World: Subterranean Ecosystems, Elsevier, Amsterdam. Google Scholar

  51. V. Sbordoni , G. Allegrucci , D. Cesaroni 2005. Population structure, pp. 447–455. In: D. Culver , W. White (Eds), Encyclopedia of Caves, Elsevier, San Diego, Ca, USA. Google Scholar

  52. A.G. Sharov 1971. Phylogeny of the Orthopteroidea. ( B. B. Rodendorf , ed.; translated from the Russian and published for the Smithsonian Institution and the National Science Foundation, Washington, D.C., by the Israel Program for Scientific Translations. Jerusalem, 1971; originally published in: Tr. Palentol. Inst. Akad. Nauk SSSR, vol. 118, 1968). 251 pp. Google Scholar

  53. C. Simon , F. Frati , A. Beckenbach , B. Crespi , H. Liu , P. Flook 1994. Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals Entomological Society of America 87: 651–701. Google Scholar

  54. J.D. Thompson , T.J. Gibson , F. Plewniak , F. Jeanmougin , D.G. Higgins 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24: 4876–4882. Google Scholar

  55. S.A. Trewick 2000. Molecular evidence for dispersal rather than vicariance as the source of flightless insects on the Chatham Islands. New Zealand. Journal of Biogeography 27: 1189–1200. Google Scholar

  56. S.A Trewick , A.M Paterson , H.J. Campbell 2007. Hello New Zealand. Journal of Biogeography 34: 1–6. Google Scholar

  57. S.A. Trewick , G.C Gibb , 2010. Vicars, tramps and assembly of the New Zealand avifauna: a review of molecular phylogenetic evidence. Ibis 152: 226–253. Google Scholar

  58. Bocxlaer I. Van , K. Roelants , S.D. Biju , J. Nagaraju , F. Bossuyt 2006. Late Cretaceous vicariance in Gondwanan amphibians. PLoS One 1: 74. doi:10.1371/journal.pone.0000074 Google Scholar

  59. J.J. Veevers (Ed.) 1984. Phanerozoic Earth History of Australia. Oxford, England. Clarendon Press. Google Scholar

  60. J.J. Veevers , C.McA. Powell , B.D. Johnson 1980. Seafloor constraints on the reconstruction of Gondwanaland. Earth Planetary Science Letter 51: 435–444. Google Scholar

  61. S. Wagstaff , F. Hennion 2007. Evolution and biogeography of Lyallia and Hectorella (Portulacaceae), geographically isolated sisters from the Southern Hemisphere. Antarctic Science 19: 417–426. Google Scholar

  62. M.O. Woodbume , J.A. Case 1996. Dispersal, vicariance, and the late Cretaceous to early Tertiary land mammal biogeography from South America to Australia. Journal of Mammalian Evolution 3: 121–161. Google Scholar

Giuliana Allegrucci, Steve A. Trewick, Angela Fortunato, Gianmaria Carchini, and Valerio Sbordoni "Cave Crickets and Cave Weta (Orthoptera, Rhaphidophoridae) from the Southern End of the World: A Molecular Phylogeny Test of Biogeographical Hypotheses," Journal of Orthoptera Research 19(1), (1 July 2010). https://doi.org/10.1665/034.019.0118
Received: 30 March 2010; Accepted: 1 June 2010; Published: 1 July 2010
JOURNAL ARTICLE
10 PAGES


SHARE
ARTICLE IMPACT
Back to Top