Identification of Arthropods by Polymerase Chain Reaction as Probes for Infectious Disease Studies in Experimental Coprolites

E. Pucu; E. Lemos; T. Rozental; M. Ogrzewalska; M. Chame; J. R. Machado-Silva; D. Leles

doi:10.1645/18-136

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E. Pucu,^1,* E. Lemos,² T. Rozental,² M. Ogrzewalska,² M. Chame,³ J. R. Machado-Silva,⁴ D. Leles¹

¹1Laboratory of Parasite Molecular Biology, Department of Microbiology and Parasitology, Universidade
²2Laboratory of Hantaviruses and Rickettsiosis, FIOCRUZ, Rio de Janeiro 21040-900 Brazil.
³3Laboratory of Paleoparasitology, FIOCRUZ, Rio de Janeiro 21040-900 Brazil.
⁴4Laboratory of Helminthology Romero Lascassa Porto, Universidade do Estado do Rio de Janeiro, Rio de

^*Correspondence should be sent to E. Pucu at: elisapucu@id.uff.br.

Abstract

The study of arthropods is still scarce in paleoparasitology, especially their molecular identification. In this experimental study, we amplify DNA using a polymerase chain reaction from 2 ectoparasite species, Rhipicephalus sanguineus and Pediculus humanus capitis, in experimentally desiccated feces. This study shows perspectives for the study of the identification of arthropods in coprolites when taxonomic identification is not possible.

Citation Download Citation

E. Pucu, E. Lemos, T. Rozental, M. Ogrzewalska, M. Chame, J. R. Machado-Silva, and D. Leles "Identification of Arthropods by Polymerase Chain Reaction as Probes for Infectious Disease Studies in Experimental Coprolites," Journal of Parasitology 105(1), 133-134, (15 February 2019). https://doi.org/10.1645/18-136

Published: 15 February 2019

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