The aim of this study was to evaluate the association between a longer sedimentation time, the reading of a larger number of slides, and the collection of multiple samples on the efficiency of the spontaneous sedimentation technique. Twenty-two patients with a previous parasitological exam positive for intestinal protozoa were recruited to collect new fecal samples (3 samples per patient) before the beginning of antiparasitic treatment. All collected fecal samples were used for spontaneous sedimentation and centrifuge-flotation techniques. Of these, all 22 patients were positive based on spontaneous sedimentation, and 59.1% (13/22) based on centrifuge flotation. The number of samples and the number of slides analyzed by spontaneous sedimentation influenced the number of positive cases. The modifications applied to the spontaneous sedimentation technique increased its performance in protozoa diagnosis.
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28 March 2019
Increasing the Efficiency of Spontaneous Sedimentation for the Diagnosis of Intestinal Protozoa
Clarissa Nascimento da Silveira Raso,
Patricia Riddell Millar,
Beatriz Brener,
Danuza Pinheiro Bastos Garcia de Mattos,
Daniela Leles,
Ana Beatriz Monteiro Fonseca,
Adriana Pittella Sudré
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Journal of Parasitology
Vol. 105 • No. 2
March 2019
Vol. 105 • No. 2
March 2019
diagnosis
Human Parasites
intestinal parasites
parasite infection
protozoa
Spontaneous Sedimentation