Parasitic infection with Toxoplasma gondii is prevalent in human and animal populations worldwide. Goat production for food and fiber has increased in popularity, and consumer demand for meat and dairy products has led to higher rates of human consumption in the United States. This trend has increased the importance of assessing the public health significance of these dietary commodities. The occurrence of T. gondii and its relevance to commercial goat production for the human food market in Mississippi has not been previously addressed. This study estimated the seroprevalence of T. gondii in breeding-age goats raised for human consumption from 4 counties in southwestern Mississippi. One hundred and forty-four goat serum samples were collected between August 2007 and April 2008 from Hinds (n = 55), Adams (n = 36), Yazoo (n = 36), and Copiah (n = 17) counties. The overall seroprevalence was 12.5% (18/144) and raises concern and justification for preventative measures including periodic surveillance of goat herds and production facilities to ensure a safe food commodity and food hygiene education for consumers.