Toxoplasma gondii is a coccidian parasite capable of infecting humans and other warm-blooded animals, with approximately 30% of the world's population chronically infected. Workers who handle animals occupationally, such as veterinarians in small-animal practices, are at a higher risk of infection. Common risk factors among these workers include contact with cats and other animals in a clinical setting. For this reason, this study aimed to determine the seroprevalence of anti-T. gondii immunoglobulin (Ig)G and IgM antibodies among small-animal veterinary personnel in Veracruz, Mexico. Blood samples were collected from 133 healthy individuals and examined using an enzyme-linked immunosorbent assay. A detailed survey on work activities was conducted. The findings showed a 2.2% positive rate for specific anti-T. gondii IgG antibodies and a 0% rate for anti-T. gondii IgM antibodies, which underscores the need for further preventive educational programs to reduce exposure to T. gondii infection in this population.