A new species of Urotrema inhabits Norops oxylophus and Norops cupreus from the Area de Conservación Guanacaste, Costa Rica. The new species is most similar to Urotrema scabridum but differs by having a relatively longer esophagus, at least 10% of the total body length versus 4–8% in U. scabridum; an oral sucker width : ventral sucker width ratio less than 1 : 1 (averaging 1 : 0.76) versus 1 : 1 or greater; an oral sucker width : pharyngeal width ratio less than 1 : 0.5 (averaging 1 : 0.42) versus greater than 1 : 0.5; and by having many transversely oriented posttesticular uterine loops versus a few vertically oriented posttesticular uterine loops.