An Improved Electron Microscopic Technique for the Immunolabeling of Cryptosporidium parvum Oocysts

Mark C. Jenkins; Charles Murphy; James Trout; Ronald Fayer

doi:10.1645/GE-3539RN.1

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Mark C. Jenkins,¹ Charles Murphy,² James Trout,³ Ronald Fayer³

¹Animal Parasitic Diseases Laboratory
²Soybean Genetics Improvement Laboratory
³Environmental Microbial Safety Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705. mjenkins@anri.barc.usda.gov

Abstract

A technique was developed for immunolabeling Cryptosporidium parvum oocysts for subsequent observation by transmission electron microscopy. This method was developed to maintain architectural integrity of the oocyst wall and improve fixation of internal contents. The improved fixation and embedding method permits efficient immunolabeling of both nonexcysted and excysted C. parvum oocysts and may be applicable to other oocyst- and cyst-forming protozoa.

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Mark C. Jenkins, Charles Murphy, James Trout, and Ronald Fayer "An Improved Electron Microscopic Technique for the Immunolabeling of Cryptosporidium parvum Oocysts," Journal of Parasitology 92(2), 403-405, (1 April 2006). https://doi.org/10.1645/GE-3539RN.1

Published: 1 April 2006

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