The rove beetle, Dalotia coriaria (Kraatz) (Coleoptera: Staphylinidae), and insidious flower bug, Orius insidiosus (Say) (Hemiptera: Anthocoridae), are biological control agents used in greenhouse production systems. However, there is no information available on the effects of five newly registered insecticides: pyrethrins and canola oil, cyclaniliprole, cyclaniliprole and flonicamid, afidopyropen, and Beauveria bassiana PPRI 5339 on adult survival of these biological control agents. Consequently, two experiments were conducted under laboratory conditions in 2020 to determine the direct effects of the five insecticides on rove beetle and insidious flower bug adults. Individual rove beetle or insidious flower bug adults were placed into Petri dishes with filter paper treated with the insecticides. After 24, 48, 72, and 96 hours, the number of live and dead adults was recorded. We found that afidopyropen at 0.27 mL/500 mL and Beauveria bassiana PPRI 5339 at 0.11 and 0.50 mL/500 mL, were not directly harmful to rove beetle or insidious flower bug adults with 100% survival. However, pyrethrins and canola oil, cyclaniliprole, and cyclaniliprole and flonicamid were directly harmful to rove beetles and insidious flower bugs with ≤ 60% adult survival. Based on our results, afidopyropen and Beauveria bassiana PPRI 5339 can be integrated into biological control programs that include the use of rove beetle or insidious flower bug adults.