An examination of leaf beetle specimens (Coleoptera: Chrysomelidae) in the five largest beetle collections in Kentucky, recent inventory work in state nature preserves, and a review of the literature revealed twenty-two species of the subfamily Chrysomelinae present in Kentucky, eight of which were previously unreported for the state. Distribution maps and label data are presented for the twenty-two Kentucky species of the subfamily Chrysomelinae including spatial (state and Kentucky county records), temporal (years and months of collection in Kentucky), and plant association information. The following species are reported from Kentucky for the first time: Chrysolina cribaria (Rogers), Chrysolina quadrigemina (Suffrian), Calligrapha californica coreopsivora Brown, Calligrapha multipunctata (Say), Calligrapha pnirsa Stål, Calligrapha spiraeae (Say), Gastrophysa cyanea F. E. Melsheimer, and Phratora americana americana (Schaeffer).