Isolated cheek teeth of Sciuridae (Rodentia, Mammalia) from nine late Miocene localities in central Anatolia (Turkey) are described. The teeth represent at least 12 different species, five of which belong to the ground squirrel genus Tamias, two to the ground squirrel genus Spermophilinus, one to the flying squirrel genus Hylopetes, and two to the flying squirrel genus Pliopetaurista. One species, Tamias anatoliensis (type locality Altintaş 1), is new. An unknown genus and species of giant tree or ground squirrel is represented by one tooth. Two teeth probably form the oldest record in western Eurasia of the tree squirrel genus Sciurus. Seven of the localities that yielded Sciuridae are lacustrine deposits, two are karst fissure fills. Their estimated ages range from MN 9 to MN 13. The majority of late Miocene Sciuridae from Anatolia show affinities with European sciurids of the same period.