A bacteriological and serological study of 201 wild capybara from the llanos, State of Apure, Venezuela was made to isolate Brucella from spleen and lymph node tissues and determine the role of this rodent as a reservoir of this bacteria. Twenty-three isolations were made, eight were identified as B. abortus and 15 as B. suis by oxidative metabolic techniques. A Poly B antigen in immunodiffusion in gel test was compared with other serologic tests. There was good correlation and 58% of sera were positive. The age and sex distribution of animals from which isolations were made and serological reactors indicated that this species may be an important alternate host of Brucella spp. in Venezuela.