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1 October 1992 MOLECULAR AND GENETIC CHARACTERIZATION OF CYTOCHROME OXIDASE-NEGATIVE AEROMONAS SALMONICIDA ISOLATED FROM COHO SALMON (ONCORHYNCHUS KISUTCH)
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Abstract

Cytochrome oxidase variants of the bacterial fish pathogen, Aeromonas salmonicida, were characterized for genetic and molecular homology with cytochrome oxidase-positive isolates that typically induce furunculosis in salmonids. Protein and lipopolysaccharide moieties of the cytochrome oxidase-negative variants were similar to their typical counterparts, based on sodium-dodecyl-sulfate polyacrylamide gel electrophoresis. Pathogenicity of aberrant isolates to brook trout (Salvelinus fontinalis) was similar to typical cytochrome oxidase-positive isolates. Colorimetric deoxyribonucleic acid (DNA) hybridization in 96-well microplates yielded homology values greater than 82.5% for typical aberrant A. salmonicida isolates when photobiotinylated DNA for reference A. salmonicida 3.101 was used as a probe. The only variation of these isolates from typical A. salmonicida was a negative cytochrome oxidase reaction.

Teska, Cipriano, and Schill: MOLECULAR AND GENETIC CHARACTERIZATION OF CYTOCHROME OXIDASE-NEGATIVE AEROMONAS SALMONICIDA ISOLATED FROM COHO SALMON (ONCORHYNCHUS KISUTCH)
Jeffrey D. Teska, Rocco C. Cipriano, and William B. Schill "MOLECULAR AND GENETIC CHARACTERIZATION OF CYTOCHROME OXIDASE-NEGATIVE AEROMONAS SALMONICIDA ISOLATED FROM COHO SALMON (ONCORHYNCHUS KISUTCH)," Journal of Wildlife Diseases 28(4), 515-520, (1 October 1992). https://doi.org/10.7589/0090-3558-28.4.515
Received: 15 January 1992; Published: 1 October 1992
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