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1 April 1994 IN VITRO ISOLATION AND CULTIVATION OF A BABESIA FROM AN AMERICAN WOODLAND CARIBOU (RANGIFER TARANDUS CARIBOU)
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Abstract

A Babesia species isolated from a captive caribou (Rangifer tarandus caribou) with clinical signs of babesiosis and a circulating parasitemia was cultured in vitro. Normal adult caribou erythrocytes supported the growth of the Babesia sp., as did erythrocytes from white-tailed deer (Odocoileus virginianus). Two basal media (M-199 and RPMI-1640) and a defined medium (HL-1) each supplemented with adult bovine serum were compared. The most favorable growth of the parasite occurred in HL-1 medium with 20% adult bovine serum. The morphology of this Babesia sp. isolate shared some characteristics with B. odocoilei and B. divergens.

Holman, Petrini, Rhyan, and Wagner: IN VITRO ISOLATION AND CULTIVATION OF A BABESIA FROM AN AMERICAN WOODLAND CARIBOU (RANGIFER TARANDUS CARIBOU)
Patricia J. Holman, Kris Petrini, Jack Rhyan, and Q. G. Wagner "IN VITRO ISOLATION AND CULTIVATION OF A BABESIA FROM AN AMERICAN WOODLAND CARIBOU (RANGIFER TARANDUS CARIBOU)," Journal of Wildlife Diseases 30(2), (1 April 1994). https://doi.org/10.7589/0090-3558-30.2.195
Received: 26 October 1992; Published: 1 April 1994
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